Multiplex genome editing of microorganisms using CRISPR-Cas
| Autor: | John van der Oost, Johannes Andries Roubos, René Verwaal, Belén Adiego-Pérez, Pascale Daran-Lapujade, Paola Randazzo, Jean-Marc Daran |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microorganism
Mini Review Computational biology Biology Microbiology 03 medical and health sciences Industrial Microbiology Biotechnology and Synthetic Biology Genome editing Genetics CRISPR genome editing Multiplex CRISPR-Cas Cas9 Molecular Biology 030304 developmental biology VLAG Regulation of gene expression Gene Editing 0303 health sciences Bacteria Cas12a 030306 microbiology BacGen Gene Expression Regulation Bacterial 3. Good health multiplex cell factories Minireview CRISPR-Cas Systems |
| Zdroj: | FEMS Microbiology Letters 366 (2019) 8 FEMS Microbiology Letters FEMS Microbiology Letters, 366(8) |
| ISSN: | 0378-1097 |
| Popis: | Microbial production of chemical compounds often requires highly engineered microbial cell factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful tools for genome editing. Here, we briefly review the most frequently used CRISPR-Cas tools and describe some of their applications. We describe the progress made with respect to CRISPR-based multiplex genome editing of industrial bacteria and eukaryotic microorganisms. We also review the state of the art in terms of gene expression regulation using CRISPRi and CRISPRa. Finally, we summarize the pillars for efficient multiplexed genome editing and present our view on future developments and applications of CRISPR-Cas tools for multiplex genome editing. Current status of multiplex genome editing in bacteria and eukaryotic microorganisms using CRISPR-Cas tools. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|