A Novel Neutralizing Antibody Specific to the DE Loop of VP1 Can Inhibit EV-D68 Infection in Mice
| Autor: | Manman Chu, Zhanlong He, Qiongwen Wu, Jinxi Yang, Ruotong Ning, Bingxiang Li, Haiting Long, Huiwen Zheng, You Gao, Jingjing Wang, Hongzhe Li, Zening Yang, Longding Liu, Lei Guo, Jiaqi Li, Haijing Shi, Wenhai Yu, Heng Li, Ming Sun, Jie Song, Haitao Fan, Chen Cheng, Xing Huang, Nan Li |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Picornavirus Flaccid paralysis medicine.drug_class Immunology Virus Attachment Monoclonal antibody Virus Mice 03 medical and health sciences Enterovirus Infections medicine Animals Immunology and Allergy Amino Acid Sequence Neutralizing antibody Enterovirus Enterovirus D Human biology Antibodies Monoclonal Viral Vaccines biology.organism_classification Antibodies Neutralizing Macaca mulatta Virology Mice Inbred C57BL Rhesus macaque 030104 developmental biology Sialic Acids biology.protein Capsid Proteins Female Nasal administration Antibody medicine.symptom |
| Zdroj: | The Journal of Immunology. 201:2557-2569 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1800655 |
| Popis: | Enterovirus D68 (EV-D68) belongs to the picornavirus family and was first isolated in CA, USA, in 1962. EV-D68 can cause severe cranial nerve system damage such as flaccid paralysis and acute respiratory diseases such as pneumonia. There are currently no efficient therapeutic methods or effective prophylactics. In this study, we isolated the mAb A6-1 from an EV-D68–infected rhesus macaque (Macaca mulatta) and found that the Ab provided effective protection in EV-D68 intranasally infected suckling mice. We observed that A6-1 bound to the DE loop of EV-D68 VP1 and interfered with the interaction between the EV-D68 virus and α2,6-linked sialic acids of the host cell. The production of A6-1 and its Ab properties present a bridging study for EV-D68 vaccine design and provide a tool for analyzing the process by which Abs can inhibit EV-D68 infection. |
| Databáze: | OpenAIRE |
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