A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection
| Autor: | Marissa Chua, Chi Lok Wong, Li Xian Choo, Malini Olivo, Heather Mittman, Hann Qian Lim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
SPR biosensor
phase-intensity detection surface plasmon resonance avian influenza A H5N1 Phase (waves) 02 engineering and technology Biosensing Techniques lcsh:Chemical technology Antibodies Viral 01 natural sciences Biochemistry Article Analytical Chemistry law.invention Birds Optics law Limit of Detection Animals lcsh:TP1-1185 Electrical and Electronic Engineering Surface plasmon resonance Instrumentation Detection limit Influenza A Virus H5N1 Subtype business.industry Chemistry 010401 analytical chemistry Resonance Polarizer Surface Plasmon Resonance 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences Influenza in Birds Prism 0210 nano-technology business Biosensor Refractive index |
| Zdroj: | Sensors (Basel, Switzerland) Sensors; Volume 17; Issue 10; Pages: 2363 Sensors, Vol 17, Iss 10, p 2363 (2017) |
| ISSN: | 1424-8220 |
| Popis: | In this paper, we present a phase-intensity surface plasmon resonance (SPR) biosensor and demonstrate its use for avian influenza A (H5N1) antibody biomarker detection. The sensor probes the intensity variation produced by the steep phase response at surface plasmon excitation. The prism sensor head is fixed between a pair of polarizers with a perpendicular orientation angle and a forbidden transmission path. At SPR, a steep phase change is introduced between the p- and s-polarized light, and this rotates the polarization ellipse of the transmission beam. This allows the light at resonance to be transmitted and a corresponding intensity change to be detected. Neither time-consuming interference fringe analysis nor a phase extraction process is required. In refractive index sensing experiments, the sensor resolution was determined to be 6.3 × 10−6 refractive index values (RIU). The sensor has been further applied for H5N1 antibody biomarker detection, and the sensor resolution was determined to be 193.3 ng mL−1, compared to 1 μg mL−1 and 0.5 μg mL−1, as reported in literature for influenza antibody detection using commercial Biacore systems. It represents a 517.3% and 258.7% improvement in detection limit, respectively. With the unique features of label-free, real-time, and sensitive detection, the phase-intensity SPR biosensor has promising potential applications in influenza detection. |
| Databáze: | OpenAIRE |
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