Pitfall Flower Development and Organ Identity of Ceropegia sandersonii (Apocynaceae-Asclepiadoideae)
| Autor: | Bertie Joan van Heuven, Annemarie Heiduk, Dewi Pramanik, Barbara Gravendeel, Marlies Spaans, Nemi Dorst, Loes Gast |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pollination Ontogeny Stamen RT-PCR MADS-box genes Plant Science 010603 evolutionary biology 01 natural sciences Ceropegia sandersonii Homology (biology) Article Ceropegia 03 medical and health sciences transcriptomics vascularization Ecology Evolution Behavior and Systematics Ecology biology Apocynaceae Plant Ecology Botany Asclepiadoideae biology.organism_classification 030104 developmental biology Evolutionary biology QK1-989 micro-CT scanning SEM |
| Zdroj: | Plants, 9, 12 Plants Plants, Vol 9, Iss 1767, p 1767 (2020) Volume 9 Issue 12 Plants, 9 |
| ISSN: | 2223-7747 |
| Popis: | Deceptive Ceropegia pitfall flowers are an outstanding example of synorganized morphological complexity. Floral organs functionally synergise to trap fly-pollinators inside the fused corolla. Successful pollination requires precise positioning of flies headfirst into cavities at the gynostegium. These cavities are formed by the corona, a specialized organ of corolline and/or staminal origin. The interplay of floral organs to achieve pollination is well studied but their evolutionary origin is still unclear. We aimed to obtain more insight in the homology of the corona and therefore investigated floral anatomy, ontogeny, vascularization, and differential MADS-box gene expression in Ceropegia sandersonii using X-ray microtomography, Light and Scanning Electronic Microscopy, and RT-PCR. During 10 defined developmental phases, the corona appears in phase 7 at the base of the stamens and was not found to be vascularized. A floral reference transcriptome was generated and 14 MADS-box gene homologs, representing all major MADS-box gene classes, were identified. B- and C-class gene expression was found in mature coronas. Our results indicate staminal origin of the corona, and we propose a first ABCDE-model for floral organ identity in Ceropegia to lay the foundation for a better understanding of the molecular background of pitfall flower evolution in Apocynaceae. |
| Databáze: | OpenAIRE |
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