Psychopharmacological effects of riparin III from Aniba riparia (Nees) Mez. (Lauraceae) supported by metabolic approach and multivariate data analysis
| Autor: | Stanley Juan Chavez Gutierrez, Adriana Maria Fernandes de Oliveira Golzio, Isis Fernandes Gomes, Reinaldo Nóbrega de Almeida, Marcus Tullius Scotti, Augusto Lopes Souto, Josean Fechine Tavares, José Maria Barbosa-Filho, Marcelo Sobral da Silva, Sócrates Golzio dos Santos |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Riparia
Multivariate analysis Hydrocortisone medicine.drug_class Footprint Tyramine Urine Pharmacology Anxiolytic Open field 03 medical and health sciences Lauraceae 0302 clinical medicine medicine Animals Rats Wistar Maze Learning 030304 developmental biology 0303 health sciences biology Behavior Animal Metabolomics analysis lcsh:Other systems of medicine biology.organism_classification lcsh:RZ201-999 Rats Riparin Complementary and alternative medicine Anxiogenic Anti-Anxiety Agents Aniba riparia Benzamides Multivariate Analysis Aniba Administration Intravenous Anxiety animal model 030217 neurology & neurosurgery Biomarkers Brazil Research Article |
| Zdroj: | BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-13 (2020) BMC Complementary Medicine and Therapies |
| ISSN: | 2662-7671 |
| Popis: | Background Currently there is a high prevalence of humor disorders such as anxiety and depression throughout the world, especially concerning advanced age patients. Aniba riparia (Nees) Mez. (Lauraceae), popular known as “louro”, can be found from the Amazon through Guianas until the Andes. Previous studies have already reported the isolation of alkamide-type alkaloids such as riparin III (O-methyl-N-2,6-dyhydroxy-benzoyl tyramine) which has demonstrated anxiolytic and antidepressant-like effects in high doses by intraperitoneal administration. Methods Experimental protocol was conducted in order to analyze the anxiolytic-like effect of riparin III at lower doses by intravenous administration to Wistar rats (Rattus norvegicus) (n = 5). The experimental approach was designed to last 15 days, divided in 3 distinct periods of five days: control, anxiogenic and treatment periods. The anxiolytic-like effect was evaluated by experimental behavior tests such as open field and elevated plus-maze test, combined with urine metabolic footprint analysis. The urine was collected daily and analyzed by 1H NMR. Generated data were statistically treated by Principal Component Analysis in order to detect patterns among the distinct periods evaluated as well as biomarkers responsible for its distinction. Results It was observed on treatment group that cortisol, biomarker related to physiological stress was reduced, indicating anxiolytic-like effect of riparin III, probably through activation of 5-HT2A receptors, which was corroborated by behavioral tests. Conclusion 1H NMR urine metabolic footprint combined with multivariate data analysis have demonstrated to be an important diagnostic tool to prove the anxiolytic-like effect of riparin III in a more efficient and pragmatic way. |
| Databáze: | OpenAIRE |
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