The potential role of the lymphocyte in fetal wound healing
| Autor: | Susan K. DiSanto, Peter W. Dillon, John C. Bleacher, Vincent R. Adolph, Thomas M. Krummel |
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| Rok vydání: | 1993 |
| Předmět: |
Surgical Sponges
Pathology medicine.medical_specialty Time Factors Lymphocyte Peripheral blood mononuclear cell Fetus Transforming Growth Factor beta Fibrosis medicine Animals Humans Lymphocytes Wound Healing integumentary system business.industry General Medicine T lymphocyte medicine.disease Immunohistochemistry Recombinant Proteins Cellular Infiltrate medicine.anatomical_structure Prenatal Injuries Polyvinyl Alcohol Pediatrics Perinatology and Child Health Wounds and Injuries Surgery Rabbits Wound healing business |
| Zdroj: | Journal of Pediatric Surgery. 28:1316-1320 |
| ISSN: | 0022-3468 |
| Popis: | Wound healing is a complex process involving a number of different cell populations. Since fetal wounds contain only a small number of mononuclear cells and no fibrosis immunohistochemical techniques using monoclonal antibodies specific to T and B lymphocytes, as well as macrophages, were used to identify the mononuclear cells in fetal wounds and to compare the lymphocyte response with the adult wound healing response. Polyvinyl alcohol sponges were placed subcutaneously in fetal rabbits on day 24 of gestation (then 31 days). Adult rabbits underwent similar implantation. Implants (8 to 14 per group) were harvested 5 days later and examined by HE and trichrome staining as well as immunohistochemical staining with monoclonal antibody (Mab) specific for rabbit T and B lymphocytes. The sponges in one group of fetal rabbits were impregnated with the polypeptide growth factor, transforming growth factor-beta (TGF-beta), a known regulator of adult tissue repair. Adult wounds showed an intense inflammatory response with extensive collagen deposition; 80% of the infiltrating cellular elements were T lymphocytes. Fetal wounds were less cellular with minimal collagen deposition; 40% of the cells were T lymphocytes. The fetal wounds treated with TGF-beta were intensely cellular and fibrotic with 71% of the cellular infiltrate comprised of T lymphocytes. The increased cellularity of the TGF-beta wounds appears to be primarily the result of increased numbers of T lymphocytes. These findings demonstrate that the fetus mounts an attenuated T lymphocyte response compared to the adult. The increased response of T lymphocytes with TGF-beta suggests that the specific growth factor milieu may account for the fetal response to wounding.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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