Urinary Tamm-Horsfall protein, albumin, vitamin D-binding protein, and retinol-binding protein as early biomarkers of chronic kidney disease in dogs

Autor: José Manuel Condor Capcha, Márcia Mery Kogika, Douglas Segalla Caragelasco, Cínthia Ribas Martorelli, Camila Rodrigues, Dennis J. Chew, Lucia Andrade, Fernanda Chicharo Chacar, Talita Rojas Sanches
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
dogs
Tamm–Horsfall protein
040301 veterinary sciences
Physiology
Vitamin D-binding protein
vitamin D‐binding protein
Urinary system
Urine
urologic and male genital diseases
Kidney
PROTEINÚRIA
0403 veterinary science
03 medical and health sciences
Physiology (medical)
Internal medicine
Chronic kidney disease
Uromodulin
medicine
Animals
Renal Insufficiency
Chronic
Tamm‐Horsfall protein
Original Research
Proteinuria
biology
business.industry
Vitamin D-Binding Protein
Albumin
04 agricultural and veterinary sciences
medicine.disease
female genital diseases and pregnancy complications
Renal Conditions
Disorders and Treatments
Retinol-Binding Proteins
Retinol binding protein
030104 developmental biology
Endocrinology
biology.protein
Female
medicine.symptom
business
retinol‐binding protein
Biomarkers
Kidney disease
Zdroj: Physiological Reports
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 2051-817X
Popis: Proteinuria is a marker and mediator of chronic kidney disease (CKD). In clinical practice, the urinary protein‐to‐creatinine ratio (UP/C) is of limited usefulness, because it indicates only the magnitude of proteinuria and not the origin of the loss (glomerular or tubular). The complete assessment of proteinuria includes quantitative and qualitative evaluations, both of which are required in order to optimize the therapy. In addition to measuring the UP/C, we performed SDS‐PAGE and western blotting to determine the expression of albumin, vitamin D‐binding protein (VDBP), retinol‐binding protein (RBP), and Tamm‐Horsfall protein (THP) in urine samples of 49 dogs: healthy (control) dogs (n = 9); and dogs with CKD (n = 40), stratified by stage. In the dogs with stage 3 or 4 CKD, there was a predominance of tubular proteins. Neither VDBP nor RBP was observed in the urine of the control dogs. Among the dogs with stage 1 or 2 CKD, VDBP and RBP were detected in those without proteinuria or with borderline proteinuria. The expression of urinary albumin was significantly higher in the stage 4 group than in any other group (P ≤ 0.01). In the stage 4 group, urinary THP was either undetectable or lower than in the control group (P ≤ 0.01). In conclusion, urinary VDBP and RBP might act as early markers of kidney injury, and a decrease in urinary THP could be an indicator of CKD progression.
Databáze: OpenAIRE
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