| Autor: |
Masuyo Miyake, Kentaro Miyake, Qinghong Han, Kentaro Igarashi, Kei Kawaguchi, Maryam Barangi, Tasuku Kiyuna, Norihiko Sugisawa, Takashi Higuchi, Hiromichi Oshiro, Zhiying Zhang, Sahar Razmjooei, Michael Bouvet, Itaru Endo, Robert M. Hoffman |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Biochemical and biophysical research communications. 643 |
| ISSN: |
1090-2104 |
| Popis: |
Gastric cancer is highly malignant and recalcitrant to first line chemotherapies that include 5-fluorouracil (5-FU). Cancer cells are addicted to methionine for their proliferation and survival. Methionine addiction of cancer is known as the Hoffman effect. Methionine restriction with recombinant methioninase (rMETase) has been shown to selectively starve cancer cells and has shown synergy with cytotoxic chemotherapy including 5-FU. The present study aimed to investigate the efficacy of rMETase alone and the combination with 5-FU on poorly differentiated human gastric cancer cell lines (MKN45, NUGC3, and NUGC4) in vitro and vivo. rMETase suppressed the tumor growth of 3 kinds of poorly differentiated gastric cancer cells in vitro. The fluorescence ubiquitination-based cell cycle indicator (FUCCI) demonstrated cancer cells treated with rMETase were selectively trapped in the S/G |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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