A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling

Autor: Yoshiaki Abe, Mamiko Sakata-Yanagimoto, Manabu Fujisawa, Hiroaki Miyoshi, Yasuhito Suehara, Keiichiro Hattori, Manabu Kusakabe, Tatsuhiro Sakamoto, Hidekazu Nishikii, Tran B. Nguyen, Yohei Owada, Tsuyoshi Enomoto, Aya Sawa, Hiroko Bando, Chikashi Yoshida, Rikako Tabata, Toshiki Terao, Masahiro Nakayama, Koichi Ohshima, Kensuke Usuki, Tatsuya Oda, Kosei Matsue, Shigeru Chiba
Rok vydání: 2022
Předmět:
Zdroj: Nature Cell Biology. 24:565-578
ISSN: 1476-4679
1465-7392
Popis: The activities of non-haematopoietic cells (NHCs), including mesenchymal stromal cells and endothelial cells, in lymphomas are reported to underlie lymphomagenesis. However, our understanding of lymphoma NHCs has been hampered by unexplained NHC heterogeneity, even in normal human lymph nodes (LNs). Here we constructed a single-cell transcriptome atlas of more than 100,000 NHCs collected from 27 human samples, including LNs and various nodal lymphomas, and it revealed 30 distinct subclusters, including some that were previously unrecognized. Notably, this atlas was useful for comparative analyses with lymphoma NHCs, which revealed an unanticipated landscape of subcluster-specific changes in gene expression and interaction with malignant cells in follicular lymphoma NHCs. This facilitates our understanding of stromal remodelling in lymphoma and highlights potential clinical biomarkers. Our study largely updates NHC taxonomy in human LNs and analysis of disease status, and provides a rich resource and deeper insights into LN and lymphoma biology to advance lymphoma management and therapy.
Databáze: OpenAIRE
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