Expression of Dystrophins and the Dystrophin-Associated-Protein Complex by Pituicytes in Culture
| Autor: | Ghazi Ayad, Serge Picaud, Thomas Claudepierre, Alvaro Rendon, Latifa Dorbani-Mamine, Halima Darbeida, Abdelkader Bougrid, Dominique Mornet |
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| Přispěvatelé: | Université de Lorraine (UL) |
| Rok vydání: | 2011 |
| Předmět: |
Male
musculoskeletal diseases congenital hereditary and neonatal diseases and abnormalities Utrophin [SDV]Life Sciences [q-bio] Immunofluorescence Biochemistry Dystrophin-Associated Protein Complex Dystrophin Dystroglycans Extracellular matrix 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Dystrophin-associated protein complex Pituitary Gland Posterior Laminin medicine Dystroglycan Animals Humans Protein Isoforms Rats Wistar Cells Cultured ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology medicine.diagnostic_test Gene Expression Profiling General Medicine musculoskeletal system Rats Pituicyte Blot Dystrophin-Associated Proteins biology.protein Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neurochemical Research Neurochemical Research, Springer Verlag, 2011, 36 (8), pp.1407-1416. ⟨10.1007/s11064-011-0466-6⟩ |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-011-0466-6 |
| Popis: | The dystrophin-associated-protein complex (DAPC) has been extensively characterized in the central nervous system where it is localized both in neuronal and glial cells. Few studies have characterized this complex in the neurohypophysis. To further study this complex in pituicytes, the resident astroglia of the neurophypophysis, we used adult pituicyte cultures and determined the expression and localization of dystrophins/utrophins and the DAPC by RT-PCR, western blotting and immunofluorescence. Our data show that the pituicytes express dystrophins, utrophins and several members of the DAPC including dystroglycans, δ-, γ-sarcoglycans, α-dystrobrevin-1 and α1-syntrophin. Double immunofluorescence analysis shows that laminin colocalizes with dystroglycan, suggesting that similarly to muscle and astrocytes, the DAPC interacts with the extracellular matrix in pituicytes. Collectively these findings show that dystrophins/utrophins and members of the DAPC are expressed in pituicytes where they may form multiprotein complexes and play a role in the retraction-reinsertion of pituicyte endfeet during specific physiological conditions. |
| Databáze: | OpenAIRE |
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