Comprehensive Analytical Comparison of Strategies Used for Small Molecule Aptamer Evaluation

Autor:	Annamaria Ruscito, Erin M. McConnell, Kayla Hill, Annalisa De Girolamo, Maria C. DeRosa, Stefania Valenzano, McKenzie Smith, Michelangelo Pascale, Ranganathan Velu, Nadine R. Frost, Maureen McKeague
Rok vydání:	2015
Předmět:	Chemistry Aptamer SELEX Aptamer Technique High selectivity aptamer Ochratoxin A Nanotechnology Computational biology Aptamers Nucleotide Affinity binding Small molecule Chemistry Techniques Analytical 3. Good health Analytical Chemistry Molecular recognition binding affinity Carrier Proteins
Zdroj:	Analytical chemistry (Wash.) 87 (2015): 8608–8612. doi:10.1021/acs.analchem.5b02102 info:cnr-pdr/source/autori:McKeague, Maureen; De Girolamo, Annalisa; Valenzano, Stefania; Pascale, Michelangelo; Ruscito, Annamaria; Velu, Ranganathan; Frost, Nadine R.; Hill, Kayla; Smith, McKenzie; McConnell, Erin M.; DeRosa, Maria C./titolo:Comprehensive Analytical Comparison of Strategies Used for Small Molecule Aptamer Evaluation/doi:10.1021%2Facs.analchem.5b02102/rivista:Analytical chemistry (Wash.)/anno:2015/pagina_da:8608/pagina_a:8612/intervallo_pagine:8608–8612/volume:87
ISSN:	1520-6882 0003-2700
DOI:	10.1021/acs.analchem.5b02102
Popis:	Nucleic acid aptamers are versatile molecular recognition agents that bind to their targets with high selectivity and affinity. The past few years have seen a dramatic increase in aptamer development and interest for diagnostic and therapeutic applications. As the applications for aptamers expand, the need for a more standardized, stringent, and informative characterization and validation methodology increases. Here we performed a comprehensive analysis of a panel of conventional affinity binding assays using a suite of aptamers for the small molecule target ochratoxin A (OTA). Our results highlight inconsistency between conventional affinity assays and the need for multiple characterization strategies. To mitigate some of the challenges revealed in our head-to-head comparison of aptamer binding assays, we further developed and evaluated a set of novel strategies that facilitate efficient screening and characterization of aptamers in solution. Finally, we provide a workflow that permits rapid and robust screening, characterization, and functional verification of aptamers thus improving their development and integration into novel applications.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b8301cb8b50bae9f0b2a148851ad0f7 https://doi.org/10.1021/acs.analchem.5b02102 Zobrazit plný text záznamu