Diagnosing Human Anisakiasis: Recombinant Ani s 1 and Ani s 7 Allergens versus the UniCAP 100 Fluorescence Enzyme Immunoassay
| Autor: | Humberto González-Díaz, A. M. Anadón, Tomas Chivato, M. Rodero, F. Romarís, Florencio M. Ubeira, Carmen Cuéllar, M. T. Gárate, Esperanza Rodríguez |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Microbiology (medical) Adolescent Clinical Biochemistry Immunology Antibodies Helminth Biology Anisakiasis Immunoglobulin E Sensitivity and Specificity Anisakis law.invention Serology Immunoenzyme Techniques Young Adult Antigen law medicine Animals Humans Immunology and Allergy Diagnostic Errors Child Aged Aged 80 and over chemistry.chemical_classification medicine.diagnostic_test Allergens Middle Aged biology.organism_classification Recombinant Proteins Enzyme Enfermedades parasitarias chemistry Antigens Helminth Child Preschool Immunoassay Recombinant DNA biology.protein Female Microbial Immunology Antibody |
| Zdroj: | CEU Repositorio Institucional Fundación Universitaria San Pablo CEU (FUSPCEU) |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.00443-09 |
| Popis: | Commercially available serological methods for serodiagnosis of human anisakiasis either are poorly specific or do not include some of the most relevantAnisakisallergens. The use of selected recombinant allergens may improve serodiagnosis. To compare the diagnostic and clinical values of enzyme-linked immunosorbent assay (ELISA) methods based on Ani s 1 and Ani s 7 recombinant allergens and of the UniCAP 100 fluorescence enzyme immunoassay (CAP FEIA) system, we tested sera from 495 allergic and 25 non-food-related allergic patients. The decay in specific IgE antibodies in serum was also investigated in 15 positive patients over a period of 6 to 38 months. Considering sera that tested positive by either Ani s 1 or Ani s 7 ELISA, the CAP FEIA classified 25% of sera as falsely positive, mainly in the group of patients with the lowest levels of anti-AnisakisIgE antibodies, and 1.28% of positive sera as falsely negative. Considering allergens individually, the overall sensitivities of Ani s 7 ELISA and Ani s 1 ELISA were 94% and 61%, respectively. The results also showed that anti-AnisakisIgE antibodies can be detected in serum for longer with Ani s 1 ELISA than with Ani s 7 ELISA and CAP FEIA (P< 0.01). Our findings suggest that ELISA methods with Ani s 7 and Ani s 1 allergens as targets of IgE antibodies are currently the best option for serodiagnosis of human anisakiasis, combining specificity and sensitivity. The different persistence of anti-Ani s 1 and anti-Ani s 7 antibodies in serum may help clinicians to distinguish between recent and oldAnisakisinfections. |
| Databáze: | OpenAIRE |
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