Extracorporeal photochemotherapy induces arginase 1 in patients with graft versus host disease

Autor: Dalil Hannani, A. de la Torre, M.P. Vasson, N. Goncalves-Mendes, M.-C. Farges, Etienne Merlin, H. Laroye, Justyna Kanold, François Demeocq
Přispěvatelé: CIC Clermont Ferrand, Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand-Centre de Pharmacologie Clinique, Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Centre d'Investigation Clinique [CHU Clermont-Ferrand] (CIC 1405), Institut National de la Santé et de la Recherche Médicale (INSERM)-Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), CHU Clermont-Ferrand-CHU Clermont-Ferrand, Hannani, Dalil
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Male
Ornithine
Arginine
Graft vs Host Disease
Nitric Oxide Synthase Type II
Apoptosis
Immune tolerance
chemistry.chemical_compound
0302 clinical medicine
fluids and secretions
Immunology and Allergy
Child
Cells
Cultured
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
Middle Aged
3. Good health
Interleukin-10
Arginase
Interleukin 10
Enzyme Induction
Photopheresis
Cytokines
Amino acids
Tumor necrosis factor alpha
Female
[SDV.IMM.IMM] Life Sciences [q-bio]/Immunology/Immunotherapy
Adult
Adolescent
Bone marrow transplantation
Immunology
education
Biology
Nitric Oxide
03 medical and health sciences
Interferon-gamma
Immune system
medicine
Immune Tolerance
Humans
RNA
Messenger
030304 developmental biology
Transplantation
Tumor Necrosis Factor-alpha
PBMC Arginine
[SDV.IMM.IMM]Life Sciences [q-bio]/Immunology/Immunotherapy
medicine.disease
Graft-versus-host disease
chemistry
ECP
030215 immunology
Zdroj: Transplant Immunology
Transplant Immunology, Elsevier, 2011, 24 (2), pp.100-106. ⟨10.1016/j.trim.2010.10.007⟩
Transplant Immunology, 2011, 24 (2), pp.100-106. ⟨10.1016/j.trim.2010.10.007⟩
ISSN: 0966-3274
DOI: 10.1016/j.trim.2010.10.007⟩
Popis: International audience; The benefits of extracorporeal photochemotherapy (ECP; psoralen and UVA exposure of blood mononuclear cells) in graft-versus-host-disease (GVHD) are well-recognized, but the mechanisms of action remain elusive. As the metabolism of L-arginine in immune cells is known to play a role in immune tolerance, we investigated the effect of ECP on arginine metabolism, and the influence of extracellular L-arginine concentration on the response to ECP in cells from patients on therapy by ECP for a GVHD and healthy donors cultured before and after ECP in the presence of different concentrations of arginine (0, 50, 100, 200 and 1000 μmol/l). At baseline arginine was not metabolized through the same pathway in patients and donors. When cells were exposed to ECP, the production of ornithine but not NO° was enhanced, while mRNA of arginase 1 was up-regulated but not INOS. In GVHD patients, increasing arginine concentration resulted in down-regulation of IFNγ and TNFα mRNA expression, whereas IL10 was up-regulated especially at physiological plasma levels (between 0 and 100 μM). Overall, our study shows that ECP orients the metabolism of arginine toward the arginase pathway together with shifting the cytokine profile toward IL-10, providing new insights into the enigmatic mechanism of action of ECP.
Databáze: OpenAIRE
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