Holographic detection of nanoparticles using acoustically actuated nanolenses
| Autor: | Mustafa Ugur Daloglu, Jonathan M. Cooper, Andriejus Demcenko, Aydogan Ozcan, Muhammad Khalid, Aniruddha Ray, Derek Tseng, Julien Reboud |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Computer science Science Holography General Physics and Astronomy Physics::Optics 02 engineering and technology Matlab code General Biochemistry Genetics and Molecular Biology Article law.invention 03 medical and health sciences law Computer graphics (images) lcsh:Science Lenses Multidisciplinary Extramural Imaging and sensing General Chemistry Acoustics 021001 nanoscience & nanotechnology 030104 developmental biology Nanoparticles lcsh:Q 0210 nano-technology Biomedical engineering Applied optics |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-13802-1 |
| Popis: | The optical detection of nanoparticles, including viruses and bacteria, underpins many of the biological, physical and engineering sciences. However, due to their low inherent scattering, detection of these particles remains challenging, requiring complex instrumentation involving extensive sample preparation methods, especially when sensing is performed in liquid media. Here we present an easy-to-use, high-throughput, label-free and cost-effective method for detecting nanoparticles in low volumes of liquids (25 nL) on a disposable chip, using an acoustically actuated lens-free holographic system. By creating an ultrasonic standing wave in the liquid sample, placed on a low-cost glass chip, we cause deformations in a thin liquid layer (850 nm) containing the target nanoparticles (≥140 nm), resulting in the creation of localized lens-like liquid menisci. We also show that the same acoustic waves, used to create the nanolenses, can mitigate against non-specific, adventitious nanoparticle binding, without the need for complex surface chemistries acting as blocking agents. Detection of small, translucent particles is challenging due to their low inherent scattering. Here, the authors present an easy, high-throughput, label-free method for detecting nanoparticles in low volumes of liquids on a disposable chip, using an acoustically actuated lens-free holographic system. |
| Databáze: | OpenAIRE |
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