A new route to 'bifunctional' chelating agents: conversion of amino acids to analogs of ethylenedinitrilotetraacetic acid
| Autor: | Claude F. Meares, Simon M. Yeh, David G. Sherman |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Iron
Biophysics Biochemistry Coupling reaction law.invention chemistry.chemical_compound law medicine Organic chemistry Humans Chelation Amino Acids Bifunctional Molecular Biology Walden inversion Edetic Acid Serum Albumin Carbodiimide Chelating Agents chemistry.chemical_classification Chemistry Cell Biology Human serum albumin Combinatorial chemistry Amino acid Carbodiimides Asymmetric carbon medicine.drug |
| Zdroj: | Analytical biochemistry. 100(1) |
| ISSN: | 0003-2697 |
| Popis: | Via the amides, α-amino acids can be converted to analogs of ethylenedinitrilotetraacetic acid, with retention of configuration at the asymmetric carbon. 1-( p -Carboxymethoxybenzyl)-ethylenedinitrilotetraacetic acid has been prepared from l -tyrosine and then coupled as the iron(III) chelate to 1,2-diaminoethane or to human serum albumin. The iron(III) chelate is effective in preventing EDTA carboxyl groups from reacting via carbodiimide coupling with amino groups. After the coupling reaction, iron is readily removed from the chelate upon reduction with ascorbate. These new procedures possess several attractive features for the use of “bifunctional” chelating agents in biophysical studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|