Secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen Sclerotinia sclerotiorum
| Autor: | Dominique Roby, Koanna Guyon, Sylvain Raffaele, Claudine Balagué |
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| Přispěvatelé: | Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Marie Curie CIG grant [334036], European Research Council [336808], French Laboratory of Excellence project 'TULIP' [ANR-10-LABX-41, ANR-11-IDEX-0002-02] |
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
effecteur Candidate gene Arabidopsis thaliana [SDV]Life Sciences [q-bio] Plant disease resistance 01 natural sciences Microbiology Fungal Proteins secrétome 03 medical and health sciences Sclerotinia sclerotiorum. Effectors Ascomycota Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Sclerotinia sclerotiorum Effectors Gene expression Secretome Necrotrophic fungal Pathogen sclerotinia sclérotiorum Gene 030304 developmental biology parasite nécrotrophe 0303 health sciences Fungal protein biology Effector Plants biology.organism_classification 3. Good health DNA microarray agent pathogène expression des gènes Research Article 010606 plant biology & botany Biotechnology |
| Zdroj: | BMC Genomics BMC Genomics, BioMed Central, 2014, 15, ⟨10.1186/1471-2164-15-336⟩ BMC Genomics (15), . (2014) |
| ISSN: | 1471-2164 |
| Popis: | Background The white mold fungus Sclerotinia sclerotiorum is a devastating necrotrophic plant pathogen with a remarkably broad host range. The interaction of necrotrophs with their hosts is more complex than initially thought, and still poorly understood. Results We combined bioinformatics approaches to determine the repertoire of S. sclerotiorum effector candidates and conducted detailed sequence and expression analyses on selected candidates. We identified 486 S. sclerotiorum secreted protein genes expressed in planta, many of which have no predicted enzymatic activity and may be involved in the interaction between the fungus and its hosts. We focused on those showing (i) protein domains and motifs found in known fungal effectors, (ii) signatures of positive selection, (iii) recent gene duplication, or (iv) being S. sclerotiorum-specific. We identified 78 effector candidates based on these properties. We analyzed the expression pattern of 16 representative effector candidate genes on four host plants and revealed diverse expression patterns. Conclusions These results reveal diverse predicted functions and expression patterns in the repertoire of S. sclerotiorum effector candidates. They will facilitate the functional analysis of fungal pathogenicity determinants and should prove useful in the search for plant quantitative disease resistance components active against the white mold. Electronic supplementary material The online version of this article (doi:10.1186/1471-2164-15-336) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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