Human urocortin II, a new CRF-related peptide, displays selective CRF(2)-mediated action on gastric transit in rats
| Autor: | Paul R. Saunders, Wylie Vale, Mulugeta Million, Jean Rivier, Yvette Taché, Celine Maillot |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Restraint Physical endocrine system medicine.medical_specialty Physiology Colon Corticotropin-Releasing Hormone Peptide Receptors Corticotropin-Releasing Hormone Rats Sprague-Dawley Stress Physiological Physiology (medical) Internal medicine medicine Animals Humans Pyrroles Receptor Urocortins Urocortin chemistry.chemical_classification Mammals Hepatology Gastric emptying business.industry CP-154 526 digestive oral and skin physiology Gastroenterology Peptide Fragments Rats Endocrinology Pyrimidines Urocortin II chemistry Gastric Emptying business hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | American journal of physiology. Gastrointestinal and liver physiology. 282(1) |
| ISSN: | 0193-1857 |
| Popis: | Human urocortin (hUcn) II is a new member of the corticotropin-releasing factor (CRF) family that selectively binds to the CRF2receptor. We investigated the CRF receptors involved in mediating the effects of hUcn II and human/rat CRF (h/rCRF) on gut transit. Gastric emptying, 4 h after a solid meal, and distal colonic transit (bead expulsion time) were monitored simultaneously in conscious rats. CRF antagonists were given subcutaneously 30 min before intravenous injection of peptides or partial restraint (for 90 min). hUcn II (3 or 10 μg/kg iv) inhibited gastric emptying (by 45% and 55%, respectively) and did not influence distal colonic transit. The CRF2 peptide antagonist astressin2-B blocked hUcn II action. h/rCRF, rat Ucn, and restraint delayed gastric emptying while accelerating distal colonic transit. The gastric response to intravenous h/rCRF and restraint was blocked by the CRF2 antagonist but not by the CRF1 antagonist CP-154,526, whereas the colonic response was blocked only by CP-154,526. None of the CRF antagonists influenced postprandial gut transit. These data show that intravenous h/rCRF and restraint stress-induced delayed gastric emptying involve CRF2 whereas stimulation of distal colonic transit involves CRF1. The distinct profile of hUcn II, only on gastric transit, is linked to its CRF2 selectivity. |
| Databáze: | OpenAIRE |
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