Molecular and Physiological Properties Associated with Zebra Complex Disease in Potatoes and Its Relation with Candidatus Liberibacter Contents in Psyllid Vectors

Autor: Olivia Duncan, T. Erik Mirkov, Veria Y. Alvarado, Denis Odokonyero, Herman B. Scholthof
Rok vydání: 2012
Předmět:
Candidatus Liberibacter
Gene Expression
lcsh:Medicine
Plant Science
Plant Genetics
Biochemistry
Cyclophilins
Plant Microbiology
Molecular Cell Biology
lcsh:Science
Plant Proteins
Plant Growth and Development
chemistry.chemical_classification
education.field_of_study
Multidisciplinary
Plant Stems
Ecology
biology
Plant Biochemistry
food and beverages
Plant physiology
Starch
Agriculture
Hemiptera
Catechol Oxidase
Research Article
Bactericera cockerelli
Population
Plant Pathogens
Crops
Microbiology
Rhizobiaceae
Plant-Environment Interactions
Botany
Genetics
Animals
Storage protein
Catechol oxidase
education
Biology
Plant Diseases
Solanum tuberosum
Crop Genetics
Plant Ecology
lcsh:R
fungi
Crop Diseases
Plant Pathology
biology.organism_classification
Insect Vectors
chemistry
biology.protein
lcsh:Q
Phloem
Carboxylic Ester Hydrolases
Zdroj: PLoS ONE, Vol 7, Iss 5, p e37345 (2012)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0037345
Popis: Zebra complex (ZC) disease on potatoes is associated with Candidatus Liberibacter solanacearum (CLs), an α-proteobacterium that resides in the plant phloem and is transmitted by the potato psyllid Bactericera cockerelli (Šulc). The name ZC originates from the brown striping in fried chips of infected tubers, but the whole plants also exhibit a variety of morphological features and symptoms for which the physiological or molecular basis are not understood. We determined that compared to healthy plants, stems of ZC-plants accumulate starch and more than three-fold total protein, including gene expression regulatory factors (e.g. cyclophilin) and tuber storage proteins (e.g., patatins), indicating that ZC-affected stems are reprogrammed to exhibit tuber-like physiological properties. Furthermore, the total phenolic content in ZC potato stems was elevated two-fold, and amounts of polyphenol oxidase enzyme were also high, both serving to explain the ZC-hallmark rapid brown discoloration of air-exposed damaged tissue. Newly developed quantitative and/or conventional PCR demonstrated that the percentage of psyllids in laboratory colonies containing detectable levels of CLs and its titer could fluctuate over time with effects on colony prolificacy, but presumed reproduction-associated primary endosymbiont levels remained stable. Potato plants exposed in the laboratory to psyllid populations with relatively low-CLs content survived while exposure of plants to high-CLs psyllids rapidly culminated in a lethal collapse. In conclusion, we identified plant physiological biomarkers associated with the presence of ZC and/or CLs in the vegetative potato plant tissue and determined that the titer of CLs in the psyllid population directly affects the rate of disease development in plants.
Databáze: OpenAIRE
Externí odkaz: