PrenDB, a Substrate Prediction Database to Enable Biocatalytic Use of Prenyltransferases
| Autor: | Shu-Ming Li, Peter Kolb, Florian Kindinger, Jakub Gunera |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Indoles Prenyltransferase Protein Prenylation Crystallography X-Ray computer.software_genre 01 natural sciences Biochemistry Substrate Specificity 03 medical and health sciences Prenylation Databases Protein Molecular Biology Indole test Alkyl and Aryl Transferases Database 010405 organic chemistry Chemistry Computational Biology Substrate (chemistry) Cell Biology Small molecule 0104 chemical sciences 030104 developmental biology Biocatalysis Cheminformatics Protein prenylation computer Algorithms |
| Zdroj: | Journal of Biological Chemistry. 292:4003-4021 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m116.759118 |
| Popis: | Prenyltransferases of the dimethylallyltryptophan synthase (DMATS) superfamily catalyze the attachment of prenyl or prenyl-like moieties to diverse acceptor compounds. These acceptor molecules are generally aromatic in nature and mostly indole or indole-like. Their catalytic transformation represents a major skeletal diversification step in the biosynthesis of secondary metabolites, including the indole alkaloids. DMATS enzymes thus contribute significantly to the biological and pharmacological diversity of small molecule metabolites. Understanding the substrate specificity of these enzymes could create opportunities for their biocatalytic use in preparing complex synthetic scaffolds. However, there has been no framework to achieve this in a rational way. Here, we report a chemoinformatic pipeline to enable prenyltransferase substrate prediction. We systematically catalogued 32 unique prenyltransferases and 167 unique substrates to create possible reaction matrices and compiled these data into a browsable database named PrenDB. We then used a newly developed algorithm based on molecular fragmentation to automatically extract reactive chemical epitopes. The analysis of the collected data sheds light on the thus far explored substrate space of DMATS enzymes. To assess the predictive performance of our virtual reaction extraction tool, 38 potential substrates were tested as prenyl acceptors in assays with three prenyltransferases, and we were able to detect turnover in >55% of the cases. The database, PrenDB (www.kolblab.org/prendb.php), enables the prediction of potential substrates for chemoenzymatic synthesis through substructure similarity and virtual chemical transformation techniques. It aims at making prenyltransferases and their highly regio- and stereoselective reactions accessible to the research community for integration in synthetic work flows. |
| Databáze: | OpenAIRE |
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