Long-term maintenance of in vitro cultured honeybee (Apis mellifera) embryonic cells
| Autor: | Monica Bergem, Kari Norberg, Randi. M. Aamodt |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Embryo
Nonmammalian ved/biology Ecology Cell Survival Cellular differentiation Methodology Article ved/biology.organism_classification_rank.species Cell Culture Techniques Cell Differentiation Biology Bees Embryonic stem cell In vitro Cell biology Culture Media lcsh:Biology (General) Cell culture Animals Model organism Mitosis Developmental biology lcsh:QH301-705.5 Fetal bovine serum Developmental Biology Cell Proliferation |
| Zdroj: | BMC Developmental Biology, Vol 6, Iss 1, p 17 (2006) BMC Developmental Biology |
| Popis: | Background In vitro cultivation of cells allows novel investigation of in vivo- mechanisms and is a helpful tool in developmental biology, biochemistry and functional genomics. Numerous cell lines of insect species, e.g., silkworm and mosquito, have been reported. However, this is not the case for successful long-term cultivation of cells in honeybees. Results Methods for cultivation of honeybee embryonic cells are discussed here. Pre-gastrula stage embryos were used to initiate cultures, and cells were reared on 96-wells microplates with Grace insect medium, supplemented with Fetal Bovine Serum. Cells proliferated in clusters, and maintained viable and mitotic for more than three months. Conclusion We report here, for the first time, long-term cultivation of honeybee cells. Results represent a highly useful in vitro-system for studying a model organism of increasing importance in areas such as aging, sociality and neurobiology. |
| Databáze: | OpenAIRE |
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