Lhx2 Expression in Postmitotic Cortical Neurons Initiates Assembly of the Thalamocortical Somatosensory Circuit

Autor: Hsiang-Wei Hsing, Bai-Chuang Shyu, Wei-Jen Chang, Carlos G. Briz, Shen-Ju Chou, Meng-Hsuan Wen, Zi-Hui Zhuang, Marta Nieto, Chia-Fang Wang
Rok vydání: 2016
Předmět:
0301 basic medicine
Chromatin Immunoprecipitation
Patch-Clamp Techniques
LIM-Homeodomain Proteins
Barrel (horology)
Gene Expression
Nerve Tissue Proteins
Biology
Somatosensory system
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Mice
0302 clinical medicine
Thalamus
Cell Line
Tumor
Conditional gene knockout
Animals
Promoter Regions
Genetic
lcsh:QH301-705.5
Evoked Potentials
In Situ Hybridization
Adaptor Proteins
Signal Transducing
Mice
Knockout
Neurons
dendritic asymmetry
Nuclear Receptor Subfamily 1
Group F
Member 2
Lhx2
Anatomy
Cortical neurons
Somatosensory Cortex
Barrel cortex
LIM Domain Proteins
Ephrin-A5
Mice
Inbred C57BL
layer 4 neurons
030104 developmental biology
nervous system
lcsh:Biology (General)
Ventral posterior nucleus
embryonic structures
barrel cortex
Neuroscience
030217 neurology & neurosurgery
Adenylyl Cyclases
Transcription Factors
Zdroj: Cell Reports, Vol 18, Iss 4, Pp 849-856 (2017)
ISSN: 2211-1247
Popis: Cortical neurons must be specified and make the correct connections during development. Here, we examine a mechanism initiating neuronal circuit formation in the barrel cortex, a circuit comprising thalamocortical axons (TCAs) and layer 4 (L4) neurons. When Lhx2 is selectively deleted in postmitotic cortical neurons using conditional knockout (cKO) mice, L4 neurons in the barrel cortex are initially specified but fail to form cellular barrels or develop polarized dendrites. In Lhx2 cKO mice, TCAs from the thalamic ventral posterior nucleus reach the barrel cortex but fail to further arborize to form barrels. Several activity-regulated genes and genes involved in regulating barrel formation are downregulated in the Lhx2 cKO somatosensory cortex. Among them, Btbd3, an activity-regulated gene controlling dendritic development, is a direct downstream target of Lhx2. We find that Lhx2 confers neuronal competency for activity-dependent dendritic development in L4 neurons by inducing the expression of Btbd3.
Databáze: OpenAIRE
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