Single objective tilted lightsheet for three-dimensional localization microscopy

Autor:	Hung, S., Cnossen, J.P., Fan, D., Smith, C.S., Beaurepaire, Emmanuel, Ben-Yakar, Adela, Park, YongKeun
Rok vydání:	2021
Zdroj:	Advances in Microscopic Imaging III: Proceedings Advances in Microscopic Imaging III
DOI:	10.1364/ecbo.2021.em1a.37
Popis:	Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b7ab7c4ff57040c04d18c856e434d8f https://doi.org/10.1364/ecbo.2021.em1a.37 Zobrazit plný text záznamu