Spatiotemporal expression patterns of clusterin in the mouse inner ear
| Autor: | Jeong Oh Shin, Seokwon Lee, Jinwoong Bok, Borum Sagong, Un-Kyung Kim |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Histology Fluorescent Antibody Technique Gene Expression In situ hybridization Pathology and Forensic Medicine Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation otorhinolaryngologic diseases medicine Animals Inner ear RNA Messenger Cochlea Messenger RNA biology Clusterin Gene Expression Regulation Developmental Cell Biology Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Organ of Corti Ear Inner Chaperone (protein) biology.protein Female sense organs 030217 neurology & neurosurgery |
| Zdroj: | Cell and Tissue Research. 370:89-97 |
| ISSN: | 1432-0878 0302-766X |
| Popis: | Clusterin (CLU) is an extracellular chaperone protein that is implicated in diverse physiological and pathophysiological cellular processes. CLU expression is upregulated in response to cellular stress and under certain conditions, such as neurodegenerative disease and cancer. CLU primarily functions as a chaperone that exerts cytoprotective effects by removing cellular debris and misfolded proteins and also acts as a signaling molecule that regulates pro-survival pathways. Deafness is caused by genetic factors and various extrinsic insults, including ototoxic drugs, exposure to loud sounds and aging. Considering its cytoprotectivity, CLU may also mediate cellular defense mechanisms against hearing loss due to cellular stresses. To understand the function of CLU in the inner ear, we analyze CLU expression patterns in the mouse inner ear during development and in the adult stage. Results of quantitative real-time polymerase chain reaction analysis showed that Clu mRNA levels in the inner ear were increased during embryogenesis and were constantly expressed in the adult. Detailed spatial expression patterns of Clu both in the mRNA and protein levels were analyzed throughout various developmental stages via in situ hybridization and immunofluorescence staining. Clu expression was found in specific domains of developing inner ear starting from the otocyst stage, mainly adjacent to the prosensory domain of the cochlear epithelium. In the mature inner ear, Clu expression was observed in Deiter's cells and pillar cells of the organ of Corti, outer sulcus and in basal cells of the stria vascularis in the cochlea. These specific spatiotemporal expression patterns suggest the possible roles of CLU in inner ear development and in maintaining proper hearing function. |
| Databáze: | OpenAIRE |
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