Effect of tempol on peripheral neuropathy in diet-induced obese and high-fat fed/low-dose streptozotocin-treated C57Bl6/J mice

Autor:	Lawrence J. Coppey, Alexander Obrosov, Hanna Shevalye, Mark A. Yorek
Rok vydání:	2017
Předmět:	0301 basic medicine medicine.medical_specialty Diabetic neuropathy Type 2 diabetes Diet High-Fat Biochemistry Streptozocin Article Diabetes Mellitus Experimental Cyclic N-Oxides Superoxide dismutase Mice 03 medical and health sciences 0302 clinical medicine Internal medicine Diabetes mellitus Animals Medicine Obesity biology business.industry Peripheral Nervous System Diseases General Medicine medicine.disease Streptozotocin Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure Endocrinology Peripheral neuropathy Diabetes Mellitus Type 2 biology.protein Spin Labels business Diet-induced obese 030217 neurology & neurosurgery Sensory nerve medicine.drug
Zdroj:	Free Radical Research. 51:360-367
ISSN:	1029-2470 1071-5762
Popis:	In this study, we sought to determine the efficacy of tempol on multiple neuropathic endpoints in a diet-induced obese mouse, a model of pre-diabetes, and a high-fat fed low-dose streptozotocin treated mouse, a model of type 2 diabetes. Tempol (4-hydroxy-2,2,6,6-tetramethylpiperdine -1-oxyl) is a low molecular weight, water soluble, membrane permeable, and metal-independent superoxide dismutase mimetic that has been widely used in cellular studies for the removal of intracellular and extracellular superoxide. This in vivo study was designed to be an early intervention. Fourteen weeks post-high-fat diet (6 weeks post-hyperglycemia) control, obese, and diabetic mice were divided into no treatment and treatment groups. The treated mice received tempol by gavage (150 mg/kg in water), while the untreated mice received vehicle. The diet-induced obese and the diabetic mice were maintained on the high-fat diet for the duration of the study, while the control group was maintained on the standard diet. Obesity and diabetes caused slowing of motor and sensory nerve conduction, reduction in intraepidermal nerve fiber density, thermal hypoalgesia, and mechanical allodynia. Treatment with tempol partially or completely protected obese and diabetic mice from these deficits. These studies suggest that tempol or other effective scavengers of reactive oxygen species may be a viable option for treating neural complications associated with obesity or type 2 diabetes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b7870b4da9aa43ab7fb3f7542e5fa9f https://doi.org/10.1080/10715762.2017.1315767 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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