Whole-Genome Enrichment Provides Deep Insights into Vibrio cholerae Metagenome from an African River
| Autor: | Vezzulli, L., Grande, C., Tassistro, G., Brettar, I., Höfle, M. G., Pereira, R. P. A., Mushi, D., Pallavicini, A., Vassallo, P., Pruzzo, C. |
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| Přispěvatelé: | Vezzulli, L, Grande, C., Tassistro, G., Brettar, I., Höfle, M. G., Pereira, R. P. A., Mushi, D., Pallavicini, Alberto, Vassallo, P., Pruzzo, C., Helmholtz Centre for infection research, Ihoffenstr. 7, 38124 Braunschweig, Germany. |
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
DNA Bacterial Genotyping Techniques Evolution Whole-genome enrichment 030106 microbiology Soil Science Africa Next-generation sequencing Vibrio cholerae Ecology Evolution Behavior and Systematics Ecology Biology medicine.disease_cause Genome Tanzania Vibrio cholerae Africa Next-generation sequencing Whole-genome enrichment DNA sequencing Microbiology 03 medical and health sciences Behavior and Systematics Rivers medicine Genotyping Prophage Genetics Base Sequence High-Throughput Nucleotide Sequencing Sequence Analysis DNA biology.organism_classification Ecology Evolution Behavior and Systematic Vibrio 030104 developmental biology Metagenomics Reference genome |
| Zdroj: | Microbial Ecology |
| ISSN: | 1432-184X 0095-3628 |
| DOI: | 10.1007/s00248-016-0902-x |
| Popis: | The detection and typing of Vibrio cholerae in natural aquatic environments encounter major methodological challenges related to the fact that the bacterium is often present in environmental matrices at very low abundance in nonculturable state. This study applied, for the first time to our knowledge, a whole-genome enrichment (WGE) and next-generation sequencing (NGS) approach for direct genotyping and metagenomic analysis of low abundant V. cholerae DNA ( |
| Databáze: | OpenAIRE |
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