Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group

Autor:	N Khan, D Brambilla, F. B. Hollinger, E Siwak, P S Reichelderfer, H Farzadegan, R W Coombs, C Beatty, S Rasheed, James W. Bremer, S M Hammer, Walter A. Scott, S A Fiscus
Rok vydání:	1996
Předmět:	Cryopreservation Microbiology (medical) Infectivity Microculture Time Factors Cell Cell Culture Techniques Human immunodeficiency virus (HIV) HIV Infections Biology medicine.disease_cause Virology Virus Titer medicine.anatomical_structure Cell culture HIV-1 medicine Humans Research Article
Zdroj:	Journal of Clinical Microbiology. 34:2312-2315
ISSN:	1098-660X 0095-1137
DOI:	10.1128/jcm.34.9.2312-2315.1996
Popis:	Quantitative microculture assays of cryopreserved human immunodeficiency virus type 1-infected cell suspensions and culture supernatants were compared among seven assays sites. There was no significant change in titer during 1 year of storage. The overall standard deviation for infected cell suspensions was approximately 0.8 log10 virus titer. A method for detecting deviant assay results was developed and was used to identify two donor cell preparations (n = 54) that gave consistently low titers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b7555b6578c0e9a7ba6b41c00420f4b https://doi.org/10.1128/jcm.34.9.2312-2315.1996 Zobrazit plný text záznamu