Chemical and biological evaluation of Egyptian Nile Tilapia (Oreochromis niloticas) fish scale collagen
| Autor: | Azza A. Hashem, Aiah A. El-Rashidy, Abd El-Hay G. Abu-Hussein, Shaymaa Ibrahim Habib, Nadia A. Badr, Ahmed Gad |
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| Rok vydání: | 2015 |
| Předmět: |
Fish Proteins
Protein Denaturation Biocompatibility Biocompatible Materials Biochemistry Collagen Type I Cell Line Nile tilapia X-Ray Diffraction Structural Biology Cricetinae Materials Testing Spectroscopy Fourier Transform Infrared Cell Adhesion medicine Animals Denaturation (biochemistry) Fibroblast Cell Shape Molecular Biology Chromatography biology Chemistry business.industry Biomaterial Cichlids General Medicine biology.organism_classification Culture Media Biotechnology Fish scale Oreochromis medicine.anatomical_structure business Gels Type I collagen |
| Zdroj: | International Journal of Biological Macromolecules. 79:618-626 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2015.05.019 |
| Popis: | Collagen is considered to be one of the most useful biomaterials with different medical applications. However, collagen properties differ from one source to another. The aim of this study was to extract, purify, characterize and perform preliminary biological evaluation of type I collagen from scales of Egyptian Nile Tilapia. Pepsin-solubilized collagen (PSC) was successfully prepared from Nile Tilapia fish scale waste. Lyophilized collagen was dissolved in dilute HCl to form acidic collagen solutions (ACS) which was neutralized to form gel. To confirm the biocompatibility of the produced gel, baby hamster kidney (BHK-21) fibroblast cells were seeded onto a 3D collagen gel (0.3% and 0.5%, w/v). The results of an SDS-PAGE test showed that the extracted collagens were type I collagen, with α chain composition of (α1)2α2. Thermal analysis showed that the denaturation temperature was 32 °C. X-ray diffraction (XRD) analysis and Fourier-transform infrared spectra (FTIR) showed that the extracted collagen had a triple helix structure. Active proliferation of BHK-21 cells with no signs of toxicity was evident with both collagen gel concentrations tested. The results show that Nile Tilapia scales can be an effective source of collagen extraction that could be used as a potential biomaterial in biomedical applications. |
| Databáze: | OpenAIRE |
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