Ca 2+ signals regulate mitochondrial metabolism by stimulating CREB-mediated expression of the mitochondrial Ca 2+ uniporter gene MCU
| Autor: | Jonathan Ragheb, Xue-Qian Zhang, Nicholas E. Hoffman, Santhanam Shanmughapriya, Roberto Caricchio, Brett A. Kaufman, Yandong Zhou, Sudarsan Rajan, Donald L. Gill, Tomohiro Kurosaki, Joseph Y. Cheung, Kevin J. Hines, Neelakshi R. Jog, Muniswamy Madesh, Yoshihiro Baba, Shuchi Guo, Jill E. Kolesar |
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| Rok vydání: | 2015 |
| Předmět: |
ORAI1 Protein
Biology Mitochondrion Endoplasmic Reticulum CREB Biochemistry Article Cell Line Avian Proteins Mitochondrial Proteins Mice Animals Calcium Signaling Stromal Interaction Molecule 1 Cyclic AMP Response Element-Binding Protein Uniporter Molecular Biology Calcium signaling Mice Knockout Endoplasmic reticulum STIM1 Cell Biology Inositol trisphosphate receptor Cell biology Cytosol biology.protein Calcium Channels Chickens |
| Zdroj: | Science Signaling. 8 |
| ISSN: | 1937-9145 1945-0877 |
| DOI: | 10.1126/scisignal.2005673 |
| Popis: | Cytosolic Ca2+ signals, generated through the coordinated translocation of Ca2+ across the plasma membrane (PM) and endoplasmic reticulum (ER) membrane, mediate diverse cellular responses. Mitochondrial Ca2+ is important for mitochondrial function, and when cytosolic Ca2+ concentration becomes too high, mitochondria function as cellular Ca2+ sinks. By measuring mitochondrial Ca2+ currents, we found that mitochondrial Ca2+ uptake was reduced in chicken DT40 B lymphocytes lacking either the ER-localized inositol trisphosphate receptor (IP3R), which releases Ca2+ from the ER, or Orai1 or STIM1, components of the PM-localized Ca2+ -permeable channel complex that mediates store-operated calcium entry (SOCE) in response to depletion of ER Ca2+ stores. The abundance of MCU, the pore-forming subunit of the mitochondrial Ca2+ uniporter, was reduced in cells deficient in IP3R, STIM1, or Orai1. Chromatin immunoprecipitation and promoter reporter analyses revealed that the Ca2+ -regulated transcription factor CREB (cyclic adenosine monophosphate response element-binding protein) directly bound the MCU promoter and stimulated expression. Lymphocytes deficient in IP3R, STIM1, or Orai1 exhibited altered mitochondrial metabolism, indicating that Ca2+ released from the ER and SOCE-mediated signals modulates mitochondrial function. Thus, our results showed that a transcriptional regulatory circuit involving Ca2+ -dependent activation of CREB controls the Ca2+ uptake capability of mitochondria and hence regulates mitochondrial metabolism. |
| Databáze: | OpenAIRE |
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