Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP
| Autor: | Yoshihisa Hashiguchi, Hirotomo Kato, Adela Celis Trujillo, Elsa Elvira Palacios Flores, Florencia Margarita Zúniga Saca, Abraham G. Cáceres, Jiro Arikawa, Ken Katakura, José Luis Abanto Alvares, Kumiko Yoshimatsu, Yu Koarashi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Sequence analysis Veterinary (miscellaneous) 030106 microbiology 030231 tropical medicine Leishmaniasis Cutaneous Biology Azure Stains Polymerase Chain Reaction Giemsa stain 03 medical and health sciences 0302 clinical medicine Cutaneous leishmaniasis parasitic diseases Peru medicine Humans Leishmania species Leishmania Mannose phosphate isomerase Leishmaniasis medicine.disease Virology Infectious Diseases Insect Science Parasitology Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Acta tropica. 158 |
| ISSN: | 1873-6254 |
| Popis: | A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect