Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle
| Autor: | Sergey V. Kraevskiy, Igor V. Yaminsky, Anastasia V. Popova, Evgeniy V. Dubrovin, Sergei G. Ignatov, Tatyana E. Ignatyuk, Nikolay V. Volozhantsev |
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| Rok vydání: | 2012 |
| Předmět: |
Acinetobacter baumannii
Bacterial Diseases Lysis Applied Microbiology lcsh:Medicine Microscopy Atomic Force Microbiology Bacteriophage Bacteriolysis Microbial Control Gram Negative Bacteriophages lcsh:Science Pathogen Biology Multidisciplinary biology Chemistry lcsh:R Biofilm Microbial Growth and Development biology.organism_classification Antimicrobial Bacterial Pathogens Host-Pathogen Interaction Emerging Infectious Diseases Infectious Diseases Lytic cycle Biological Control Agents Medical Microbiology Medicine lcsh:Q Bacteria Acinetobacter Infections Research Article Biotechnology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 10, p e47348 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Background: Acinetobacter baumannii is known for its ability to develop resistance to the major groups of antibiotics, form biofilms, and survive for long periods in hospital environments. The prevalence of infections caused by multidrug-resistant A. baumannii is a significant problem for the modern health care system, and application of lytic bacteriophages for controlling this pathogen may become a solution. Methodology/Principal Findings: In this study, using atomic force microscopy (AFM) and microbiological assessment we have investigated A. baumannii bacteriophage AP22, which has been recently described. AFM has revealed the morphology of bacteriophage AP22, adsorbed on the surfaces of mica, graphite and host bacterial cells. Besides, morphological changes of bacteriophage AP22-infected A. baumannii cells were characterized at different stages of the lytic cycle, from phage adsorption to the cell lysis. The phage latent period, estimated from AFM was in good agreement with that obtained by microbiological methods (40 min). Bacteriophage AP22, whose head diameter is 6261 nm and tail length is 8869 nm, was shown to disperse A. baumannii aggregates and adsorb to the bacterial surface right from the first minute of their mutual incubation at 37uC. Conclusions/Significance: High rate of bacteriophage AP22 specific adsorption and its ability to disperse bacterial aggregates make this phage very promising for biomedical antimicrobial applications. Complementing microbiological results with AFM data, we demonstrate an effective approach, which allows not only comparing independently obtained characteristics of the lytic cycle but also visualizing the infection process. |
| Databáze: | OpenAIRE |
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