Myeloid zinc finger 1 protein is a key transcription stimulating factor of PYROXD2 promoter
| Autor: | Tao Wang, Jiaojiao Chen, Xingyan Jiang, Handong Fan, Xingzhi Wang, Fang Yu, Huilin Liu, Xiaoyuan Xie |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Kruppel-Like Transcription Factors Biology 03 medical and health sciences Transcription (biology) Gene expression E2F1 Humans RNA Messenger Promoter Regions Genetic Transcription factor Sp1 transcription factor Binding Sites Liver cell Tumor Suppressor Proteins Liver Neoplasms Myeloid zinc finger 1 General Medicine Hep G2 Cells Cell biology DNA-Binding Proteins Gene Expression Regulation Neoplastic 030104 developmental biology Oncology Liver Cancer research IRF8 |
| Zdroj: | Oncology reports. 38(5) |
| ISSN: | 1791-2431 |
| Popis: | Previous studies revealed that PYROXD2 was more highly expressed in normal liver tissue and liver cell lines than in cancer tissue and cancer cell lines, which indicated that decreased PYROXD2 expression may be involved in hepatocarcinogenesis. To identify the mechanisms which regulate PYROXD2 gene transcription, we constructed a series of luciferase reporter plasmids and used them to perform luciferase‑based reporter assays with HepG2, Sk-hep1, L02 and 293T cells with the purpose of characterizing the PYROXD2 reporter region. Our results revealed that the transcription factor myeloid zinc finger 1 (MZF1) is necessary for PYROXD2 gene transcription and that it functions as a trans-activator. DNA binding assays revealed that the MZF1 protein binds to the cis-element TGGGGA located in the -320/-312 region of the PYROXD2 promoter. This promoter had a significantly enhanced activity when the MZF1 protein was overexpressed and a significantly decreased activity when the MZF1 protein expression was silenced. However, MZF1 gene expression was not significantly correlated with PYROXD2 protein expression in the samples of resected tumor tissues, which revealed that the PYROXD2 promoter transcription activity was determined by the aggregated effect of numerous transcription factors. This finding may be helpful in understanding the underlying mechanism which regulates the PYROXD2 expression. |
| Databáze: | OpenAIRE |
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