Interleukin-10 in Human Milk
| Autor: | Sadhana Chheda, Armond S. Goldman, Kimberly H. Palkowetz, Helen E. Rudloff, Roberto P. Garofalo, Frank C. Schmalstieg, David K. Rassin, Fang C Mei |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
medicine.medical_specialty medicine.medical_treatment Molecular Sequence Data Size-exclusion chromatography chemistry.chemical_compound Lactation Internal medicine medicine Humans Incubation Messenger RNA Base Sequence Milk Human biology Temperature Interleukin-10 Interleukin 10 medicine.anatomical_structure Endocrinology Cytokine chemistry Pediatrics Perinatology and Child Health biology.protein Cytokines Female Antibody Thymidine |
| Zdroj: | Pediatric Research. 37:444-449 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/00006450-199504000-00010 |
| Popis: | The concentrations of immunoreactive IL-10 in the aqueous fraction of 20 specimens of human milk obtained during the first 80 h of lactation and stored at -60 degrees C ranged from 66 to 9301 pg/mL (mean +/- SD, 3304 +/- 3127 pg/mL). IL-10 was present also in the lipid layer of milk. Gel filtration revealed that IL-10 was located in a high molecular weight fraction, where certain other cytokines in human milk have been found. In addition, immunoreactive IL-10 in milk increased after treatment with sodium taurocholate. Bioactive IL-10 was demonstrated by the finding that human milk inhibited [3H]thymidine uptake by human blood lymphocytes and that inhibition was partly overcome by concomitant incubation with antibodies to human IL-10. IL-10 mRNA but no protein product was found in cultured human mammary epithelial cells. Some IL-10 was associated with preparations of human milk leukocytes, but the data did not suggest that the cells were producing the cytokine. Bioactive IL-10 in a possible protected compartment suggests that IL-10 in human milk may have immunomodulating, antiinflammatory effects on the alimentary tract of the recipient infant. |
| Databáze: | OpenAIRE |
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