Uptake of 3-iodothyronamine hormone analogs inhibits the growth and viability of cancer cells
Autor: | Grazia Chellini, Michael Rogowski, Lauren S. Gollahon, Fariba M. Assadi-Porter |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Cell type SG‐2 General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine medicine cancer Viability assay Cytotoxicity Structural analog Research Articles metabolic substrate utilization 3‐Iodothyronamine synthetic analog Chemistry Cell growth Cancer 3‐Iodothyronamine T1AM medicine.disease 3. Good health 030104 developmental biology Cancer cell Cancer research Corrigendum 030217 neurology & neurosurgery Hormone Research Article |
Zdroj: | FEBS Open Bio |
ISSN: | 2211-5463 |
Popis: | 3-Iodothyronamine (T1AM) is a structural analog of thyroid hormone that has been demonstrated to have potent affects on numerous physiological systems. Most studies on T1AM have explored its effects in healthy functional systems; while its potential therapeutic uses and safety, and efficacy in pathological conditions are largely unknown. We sought to evaluate the effects of T1AM and its structural analog SG-2 on cancer cell growth and viability. We analyzed the cytotoxicity of these analogs on MCF7 breast cancer cells, HepG2 hepatocellular cancer cells as well as normal control cells using primary human foreskin fibroblasts and mouse preadipocytes control cells. The cytotoxicity of T1AM and SG-2 was determined by cell growth curves, and validated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell viability assays. Cellular uptake analysis was conducted using confocal microscopy. Real-time (RT)-PCR was conducted to identify gene pathways affected by SG-2 in cancer cells. The IC 50 of T1AM was approximately double the concentration of its analog SG-2 in cancer cells. Cytotoxicity studies on normal cells revealed that IC 50 concentrations of SG-2 in cancer cells had no significant impact on cell viability in these cell types. Cell-imaging experiments demonstrated rapid uptake and localization to the mitochondrial membrane. T1AM and SG-2 are able to reduce cancer cell growth and viability. These findings support the potential for use of these compounds and related analogs for their antiproliferation properties in cancer cells. |
Databáze: | OpenAIRE |
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