Time-course study of a gold nanoparticle contrast agent for cardiac-gated micro-CT imaging in mice
| Autor: | Nancy L. Ford, Samir El ketara |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Time Factors
media_common.quotation_subject 0206 medical engineering Contrast Media Metal Nanoparticles Nanoparticle 02 engineering and technology 030218 nuclear medicine & medical imaging Mice 03 medical and health sciences 0302 clinical medicine Iodinated contrast medicine Animals Contrast (vision) Tissue Distribution Four-Dimensional Computed Tomography Radionuclide Imaging General Nursing Cardiac imaging media_common Cardiac cycle Chemistry Soft tissue Heart X-Ray Microtomography 020601 biomedical engineering Mice Inbred C57BL Radiography Nanomedicine medicine.anatomical_structure Ventricle Female Gold Tomography Iodine Biomedical engineering |
| Zdroj: | Biomedical Physics & Engineering Express. 6:035025 |
| ISSN: | 2057-1976 |
| Popis: | Although micro-computed tomography (micro-CT) images have high contrast for bone or air, between soft tissues the contrast is typically low. To overcome this inherent issue, attenuating exogenous contrast agents are used to provide contrast enhancement in the vasculature and abdominal organs. The aim of this study is to measure the contrast enhancement time course for a gold nanoparticle blood-pool contrast agent and use it to perform cardiac-gated 4D micro-CT scans of the heart. Six healthy female C57BL/6 mice were anesthetized and imaged after receiving an injected dose of MVivo gold nanoparticle blood-pool contrast agent. Following the injection, we performed micro-CT scans at 0, 0.25, 0.5, 0.75, 1, 2, 4, 8, 24, 48 and 72 h. The mean CT number was measured for 7 different organs. No contrast enhancement was noticed in the bladder, kidneys or muscle during the time-course study. However, it clearly appears that the contrast enhancement is high in both right ventricle and vena cava. To perform cardiac-gated imaging, either the gold nanoparticle agent (n = 3) or an iodine-based (n = 3) contrast agent was introduced and images representing 9 phases of the cardiac cycle were obtained in 6 additional mice. A few typical cardiac parameters were measured or calculated, with similar accuracy between the gold and iodinated agents, but better visualization of structures with the gold agent. The MVivo Au contrast agent can be used for investigations of cardiac or vascular disease with a single bolus injection, with an optimal cardiac imaging window identified during the first hour after injection, demonstrating similar image quality to iodinated contrast agents and excellent measurement accuracy. Furthermore, the long-lasting contrast enhancement of up to 8 h can be very useful for scanning protocols that require longer acquisition times. |
| Databáze: | OpenAIRE |
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