Extracellular matrix alterations in the skin of patients affected by psoriasis
Autor: | Thérèse Rachell Theodoro, Luiza Keiko Matsuka Oyafuso, Mariana Fatima Muaccad Gama Wagner, Carlos D' Apparecida Santos Machado Filho, Maria Aparecida da Silva Pinhal |
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Rok vydání: | 2021 |
Předmět: |
TIMP2
Pathology medicine.medical_specialty MMP2 MMP9 Matrix metalloproteinase Extracellular matrix Psoriasis Genetic predisposition medicine Humans Heparanase Molecular Biology QH573-671 business.industry Heparanase-2 Cell Biology medicine.disease Immunohistochemistry Metalloproteinases Extracellular Matrix Syndecan-1 Cytology business Research Article |
Zdroj: | BMC Molecular and Cell Biology, Vol 22, Iss 1, Pp 1-12 (2021) BMC Molecular and Cell Biology |
ISSN: | 2661-8850 |
DOI: | 10.1186/s12860-021-00395-1 |
Popis: | Background Psoriasis is a chronic inflammatory disease dependent upon a complex interaction between genetic predisposition and immunological factors. It is characterized by skin lesions throughout the body, causing great morbidity and affecting life quality. The present study aimed to evaluate the protein and mRNA expression of heparanase-1 (HPSE), heparanase-2 (HPSE2), syndecan-1 (SYND1), metalloproteinases (MMP2, MMP9), and tissue inhibitor metalloproteinases 2 (TIMP2) in skin samples. Methods From each psoriasis patient, two samples were collected, one sample from a psoriasis plaque (n = 23) and the other sample from non-affected skin (n = 23), as well as tissue collected by blepharoplasty from control individuals (n = 18). Protein expression was investigated by immunohistochemistry, followed by digital quantification. Quantitative RT-PCR obtained mRNA expression. Statistical analyses were done, and p values Results A significant increase in protein and mRNA expression was observed in both heparanases (HPSE and HPSE2), and higher protein levels of MMP9 and TIMP2 were observed in the psoriasis plaque compared to the non-affected skin. The data point to a probable activation of MMP2 by TIMP2. Moreover, there was a significant increase in HPSE2, SYND1, MMP9, and TIMP2 in non-affected skin samples from patients with psoriasis than in the control sample (tissue obtained by individuals who do not have psoriasis). Conclusions These results show a possible correlation between the characteristic inflammatory process and alterations in the expression of the extracellular matrix in psoriasis. The increased expression of HPSE2, SYND1, MMP9, and TIMP2, even in the absence of psoriatic plaque, indicates that these molecules may be involved with extracellular matrix changes in the initial alterations the psoriatic process and may be candidates for the development of target treatments. |
Databáze: | OpenAIRE |
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