Land application of sewage sludge: Response of soil microbial communities and potential spread of antibiotic resistance
| Autor: | Mariusz Cycoń, Sławomir Sułowicz, Anna Markowicz, Kinga Bondarczuk |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
010504 meteorology & atmospheric sciences
Urease Tetracycline Health Toxicology and Mutagenesis 010501 environmental sciences Toxicology 01 natural sciences Soil Antibiotic resistance Metals Heavy medicine Soil Pollutants Organic matter 0105 earth and related environmental sciences chemistry.chemical_classification biology Sewage Microbiota Drug Resistance Microbial General Medicine biology.organism_classification Pollution Agronomy chemistry Microbial population biology biology.protein Nitrification Bacteria Sludge medicine.drug |
| Zdroj: | Environmental pollution (Barking, Essex : 1987). 271 |
| ISSN: | 1873-6424 |
| Popis: | The effect of land application of sewage sludge on soil microbial communities and the possible spread of antibiotic- and metal-resistant strains and resistance determinants were evaluated during a 720-day field experiment. Enzyme activities, the number of oligotrophic bacteria, the total number of bacteria (qPCR), functional diversity (BIOLOG) and genetic diversity (DGGE) were established. Antibiotic and metal resistance genes (ARGs, MRGs) were assessed, and the number of cultivable antibiotic- (ampicillin, tetracycline) and heavy metal- (Cd, Zn, Cu, Ni) resistant bacteria were monitored during the experiment. The application of 10 t ha−1 of sewage sludge to soil did not increase the organic matter content and caused only a temporary increase in the number of bacteria, as well as in the functional and structural biodiversity. In contrast to expectations, a general adverse effect on the tested microbial parameters was observed in the fertilized soil. The field experiment revealed a significant reduction in the activities of alkaline and acid phosphatases, urease and nitrification potential. Although sewage sludge was identified as the source of several ARGs and MRGs, these genes were not detected in the fertilized soil. The obtained results indicate that the effect of fertilization based on the recommended dose of sewage sludge was not achieved. |
| Databáze: | OpenAIRE |
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