Comparison between enzyme-linked immunosorbent assay, indirect fluorescent antibody and rapid conglutination tests in detecting antibodies against Babesia bovis
| Autor: | Denise Carneiro Lemaire, Cláudio R. Madruga, Ana Paula da Costa Marques, Raul H. Kessler, C. R. B. Leal, Flábio R. Araújo, Maria A. M. Schenk |
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| Rok vydání: | 1998 |
| Předmět: |
animal diseases
Prevalence Antibodies Protozoan Cattle Diseases Enzyme-Linked Immunosorbent Assay Immunofluorescence Sensitivity and Specificity Serology Babesiosis parasitic diseases medicine Animals Seroprevalence Fluorescent Antibody Technique Indirect Direct fluorescent antibody chemistry.chemical_classification General Veterinary biology medicine.diagnostic_test Complement Fixation Tests Babesia bovis General Medicine bacterial infections and mycoses biology.organism_classification Virology Enzyme chemistry biology.protein Cattle Female Parasitology Antibody human activities Brazil |
| Zdroj: | Veterinary Parasitology. 74:101-108 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/s0304-4017(97)00171-4 |
| Popis: | The performance of an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFAT) and a rapid conglutination test (RCT) for the detection of antibodies against Babesia bovis, was evaluated with 462 cattle sera from Bahia State; Brazil. The results showed a concordance of 96.6% between the ELISA and IFAT, 90.5% between the ELISA and RCT, and 91.8% between the IFAT and RCT. Although the prevalence rates determined by ELISA (97.2%) and IFAT (96.8%) were higher than that indicated by the RCT (92.9%), performances of the three serological tests were very similar and characterized the region studied as enzootically stable to B. bovis. |
| Databáze: | OpenAIRE |
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