Electron-microscopic, cytochemical and biochemical studies of acetylcholinesterase and butyrylcholinesterase activity in muscle of dystrophic mice
| Autor: | Leont. Kremzner, Virginia M. Tennyson, Armand F. Miranda |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
medicine.medical_specialty
Aché Sarcoplasm Connective tissue Microtubules Mice chemistry.chemical_compound Internal medicine medicine Animals Cholinesterases Myocyte chemistry.chemical_classification biology Muscles Muscular Dystrophy Animal Acetylcholinesterase Enzyme assay language.human_language Cell biology Enzyme Endocrinology medicine.anatomical_structure Neurology chemistry Butyrylcholinesterase Nerve Degeneration Synapses biology.protein language Neurology (clinical) Reticulum |
| Zdroj: | Journal of the Neurological Sciences. 25:309-332 |
| ISSN: | 0022-510X |
| DOI: | 10.1016/0022-510x(75)90154-9 |
| Popis: | We have studied extrajunctional muscle of control and dystrophic mice by electron microscopic-cytochemistry and radiometric assay. We have found both a soluble and particulate AChE activity, which is similar proportionally in control and dystrophic muscle. The particulate AChE activity is probably due to the enzyme localized in the sarcotubular system. These sites are more numerous in muscle adjacent to the motor end-plate than in distally located extrajunctional muscle, and are increased markedly in the dystrophic mouse. Myoblasts and small muscle fibers in the dystrophic mouse also have AChE activity in the reticulum similar to fetal muscle. The soluble AChE activity identified radiometrically may represent those sites exhibiting random cytochemical end-product, such as some muscle nuclei, satellite cells, myogenic mononuclear cells in the connective tissue, and degenerating axonal boutons no longer associated with junctional folds of muscle. Enzyme activity is present in degenerating fibers, but it is randomly dispersed in the sarcoplasm rather than membrane-bound. AChE activity has not been found in debris of completely necrotic muscle. BuChE activity is higher and the number of BuChE-active sites in the sarcotubular system adjacent to the motor end-plates is greater in dystrophic muscle than in control muscle. |
| Databáze: | OpenAIRE |
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