Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species
| Autor: | Lennart Eschen-Lippold, Jarosław Poznański, Patrycja Zembek, Aleksandra Danilecka, Marta Benicka, Magdalena Krzymowska, Marcin Piechocki, Jakub Kwiatkowski, Rafal Hoser, Wojciech Rymaszewski, Karolina Morgiewicz, Justin Lee, Izabela Barymow-Filoniuk, Jacek Hennig, Marta Grech-Baran |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genetics Proteases biology Effector fungi HopR1 Pseudomonas syringae food and beverages Nicotiana benthamiana Virulence Plant Science lcsh:Plant culture biology.organism_classification virulence TTSS effectors 03 medical and health sciences 030104 developmental biology Susceptible individual Gene cluster HopQ1 lcsh:SB1-1110 Original Research Nicotiana |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 9 (2018) |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2018.00978 |
| Popis: | Pseudomonas syringae employs a battery of type three secretion effectors to subvert plant immune responses. In turn, plants have developed receptors that recognize some of the bacterial effectors. Two strain-specific HopQ1 effector variants (for Hrp outer protein Q) from the pathovars phaseolicola 1448A (Pph) and tomato DC3000 (Pto) showed considerable differences in their ability to evoke disease symptoms in Nicotiana benthamiana. Surprisingly, the variants differ by only six amino acids located mostly in the N-terminal disordered region of HopQ1. We found that the presence of serine 87 and leucine 91 renders PtoHopQ1 susceptible to N-terminal processing by plant proteases. Substitutions at these two positions did not strongly affect PtoHopQ1 virulence properties in a susceptible host but they reduced bacterial growth and accelerated onset of cell death in a resistant host, suggesting that N-terminal mutations rendered PtoHopQ1 susceptible to processing in planta and, thus, represent a mechanism of recognition avoidance. Furthermore, we found that co-expression of HopR1, another effector encoded within the same gene cluster masks HopQ1 recognition in a strain-dependent manner. Together, these data suggest that HopQ1 is under high host-pathogen co-evolutionary selection pressure and P. syringae may have evolved differential effector processing or masking as two independent strategies to evade HopQ1 recognition, thus revealing another level of complexity in plant – microbe interactions. |
| Databáze: | OpenAIRE |
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