Inactivation of Germline Mutant APC Alleles by Attenuated Somatic Mutations: A Molecular Genetic Mechanism for Attenuated Familial Adenomatous Polyposis
| Autor: | Li Kuo Su, Yi Qi, Weizhe Yao, Christopher J. Barnes, Gideon Steinbach, Patrick M. Lynch |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Genes APC Beta-catenin Adolescent Genotype Colorectal cancer Adenomatous polyposis coli DNA Mutational Analysis Population Germline Familial adenomatous polyposis 03 medical and health sciences Suppression Genetic 0302 clinical medicine Germline mutation Genetics medicine Humans Genetic Predisposition to Disease Genetics(clinical) RNA Messenger Codon education Alleles Germ-Line Mutation beta Catenin Genetics (clinical) 030304 developmental biology 0303 health sciences education.field_of_study biology Articles Exons Middle Aged medicine.disease Molecular biology 3. Good health Gene Expression Regulation Neoplastic Cytoskeletal Proteins Phenotype Adenomatous Polyposis Coli Attenuated familial adenomatous polyposis 030220 oncology & carcinogenesis Trans-Activators biology.protein |
| Zdroj: | The American Journal of Human Genetics. 67:582-590 |
| ISSN: | 0002-9297 |
| DOI: | 10.1086/303058 |
| Popis: | Germline mutations of the adenomatous polyposis coli ( APC ) tumor-suppressor gene result in familial adenomatous polyposis (FAP). Patients with FAP typically develop hundreds to thousands of benign colorectal tumors and early-onset colorectal cancer. A subset of germline APC mutations results in an attenuated FAP (AFAP) phenotype, in which patients develop fewer tumors and develop them at an older age. Although a genotype-phenotype correlation between the locations of APC germline mutations and the development of AFAP has been well documented, the mechanism for AFAP has not been well defined. We investigated the mechanism for AFAP in patients carrying a mutant APC allele ( APC AS9 ) that has a mutation in the alternatively spliced region of exon 9. APC AS9 was found to down-regulate β-catenin–regulated transcription, the major tumor-suppressor function of APC, as did the wild-type APC. Mutation analysis showed that both APC AS9 and the wild-type APC alleles were somatically mutated in most colorectal tumors from these patients. Functional analysis showed that 4666insA, a common somatic mutation in APC AS9 in these tumors, did not inactivate the wild-type APC. Our results indicate that carriers of APC AS9 develop fewer colorectal tumors than do typical patients with FAP because somatic inactivation of both APC alleles is necessary for colorectal tumorigenesis. However, these patients develop colorectal tumors more frequently than does the general population because APC AS9 is inactivated by mutations that do not inactivate the wild-type APC. |
| Databáze: | OpenAIRE |
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