Sodium-dependent, concentrative nucleoside transport in cultured intestinal epithelial cells
| Autor: | Alan R. P. Paterson, Ewa S. Jakobs |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Cell type
Sodium Biophysics chemistry.chemical_element Biology digestive system Biochemistry Epithelium Intestinal mucosa Thioinosine medicine Animals Intestinal Mucosa Inosine Molecular Biology Cells Cultured Transporter Biological Transport Nucleosides Cell Biology Rats chemistry Efflux Nucleoside Formycins medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 140(3) |
| ISSN: | 0006-291X |
| Popis: | In a simple salts medium, monolayers of IEC-6 intestinal cells achieved concentrations of unmetabolized formycin B (an analog of inosine) about 6-fold higher than in the medium. Rates of formycin B influx were a saturable function of Na+ concentrations in the medium. Although IEC-6 cells possess sites with high affinity for nitrobenzylthioinosine, a potent inhibitor of equilibrative (facilitated diffusion) nucleoside transport systems in certain cell types, the inhibitor had only minor effects on formycin B uptake in IEC-6 cells, but reduced efflux of the analog from these cells. These findings indicate the joint presence in IEC-6 cells of nucleoside transporters of two types, one that is concentrative and Na+-dependent, and another that is sensitive to nitrobenzylthioinosine and apparently equilibrative. |
| Databáze: | OpenAIRE |
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