Potent anti-cancer activity of Alnus nitida against lung cancer cells; in vitro and in vivo studies
| Autor: | Chao Yan, Moniba Sajid, Muhammad Rashid Khan, Siva Bharath Merugu, Dawei Li, Hema Negi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell cycle checkpoint Lung Neoplasms Cell Survival Apoptosis Alnus nitida RM1-950 Alnus 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Cyclin D1 In vivo medicine Animals Humans Lung cancer Pharmacology Dose-Response Relationship Drug Chemistry Plant Extracts Cancer General Medicine medicine.disease Colony survival Molecular biology Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays respiratory tract diseases Mice Inbred C57BL 030104 developmental biology A549 Cells 030220 oncology & carcinogenesis Cancer cell Therapeutics. Pharmacology Growth inhibition Actin fibers |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 110, Iss, Pp 254-264 (2019) |
| ISSN: | 0753-3322 |
| Popis: | Alnus nitida is used for multiple disorders in norther areas of Pakistan. In this study we have evaluated methanol extract of leaf (ANL) and stem bark (ANB) of A. nitida against two lung cancer cell lines; A-549 and H460 (Human non-small lung cancer cell lines) during in vitro assays for growth inhibition. Treatment with ANL and ANB markedly inhibited the growth of both cancer cell lines. Exposure of A-549 and H460 cancer cell lines to ANL and ANB inhibited cell survival, colony growth and migration of cells. Further, treatment of A-549 and H460 with ANL and ANB indicated alteration in actin fibers after staining with rhodamine-phalloidin. Both extracts cause shrinkage and cell cycle arrest during G1 phase. Treatment of A-549 and H460 cancer cells with ANL and ANB repressed the expression of anti-apoptotic proteins Bcl-2 and Bcl-xL along with downregulation of NFκB, cyclin D1 and PI3-K protein. In addition, intraperitoneal injection of ANL and ANB (10 mg/kg bw and 20 mg/kg bw) to C57BL/6 J mice implanted with B16F10 (Mouse melanoma cancer cell line) cells significantly (p |
| Databáze: | OpenAIRE |
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