Transcriptional Responses of Human Aortic Endothelial Cells to Nanoconstructs Used in Biomedical Applications
| Autor: | Alexander Malugin, Hamidreza Ghandehari, Giridhar Thiagarajan, Philip J. Moos, Heather Herd, Matthew Honeggar |
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| Rok vydání: | 2013 |
| Předmět: |
Dendrimers
Chemistry Silicon dioxide Cell Cycle Endothelial Cells Pharmaceutical Science Nanotechnology Cell cycle Silicon Dioxide Cell Cycle Gene Article Transcriptome chemistry.chemical_compound Dendrimer Drug Discovery Drug delivery Toxicity Polyamines Biophysics Humans Nanoparticles Molecular Medicine Cytotoxicity Cells Cultured |
| Zdroj: | Molecular Pharmaceutics. 10:3242-3252 |
| ISSN: | 1543-8392 1543-8384 |
| Popis: | Understanding the potential toxicities of manufactured nanoconstructs used for drug delivery and biomedical applications may help improve their safety. We sought to determine if surface-modified silica nanoparticles and poly(amido amine) dendrimers elicit genotoxic responses on vascular endothelial cells. The nanoconstructs utilized in this study had a distinct geometry (spheres vs worms) and surface charge, which were used to evaluate the contributions of these parameters to any potential adverse effects of these materials. Time-dependent cytotoxicity was found for surfaced-functionalized but geometrically distinct silica materials, while amine-terminated dendrimers displayed time-independent cytotoxicity and carboxylated dendrimers were nontoxic in our assays. Transcriptomic evaluation of human aortic endothelial cell (HAEC) responses indicated time-dependent gene induction following silica exposure, consisting of cell cycle gene repression and pro-inflammatory gene induction. However, the dendrimers did not induce genomic toxicity, despite displaying general cytotoxicity. |
| Databáze: | OpenAIRE |
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