Evaluation of Sensitivity and Specificity of a Standardized Procedure Using Different Reagents for the Detection of Lupus Anticoagulants

Autor:	C Caron, Pierre Sie, Jeanne-Yvonne Borg, A Derlon, J Sampol, D. de Prost, J. Goudemand
Rok vydání:	1997
Předmět:	Lupus anticoagulant Systemic lupus erythematosus medicine.diagnostic_test business.industry Hematology medicine.disease Molecular biology Hemostasis Reagent Immunology Oral anticoagulant medicine Platelet Positive test business Partial thromboplastin time
Zdroj:	Scopus-Elsevier
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1655965
Popis:	SummaryThis study was designed to test the sensitivity and specificity of a combination of 3 phospholipid-dependent assays performed with various reagents, for the detection of lupus anticoagulant (LA). Plasmas containing an LA (n = 56) or displaying various confounding pathologies [58 intrinsic pathway factor deficiencies, 9 factor VIII inhibitors, 28 plasmas from patients treated with an oral anticoagulant (OAC)] were selected. In a first step, the efficiency of each assay and reagent was assessed using the Receiving Operating Characteristic (ROC) method. Optimal cut-offs providing both sensitivity and specificity ≥80% were determined. The APTT assay and most of the phospholipid neutralization assays failed to discriminate factor VIII inhibitors from LA. In a second step, using the optimal cut-offs determined above, the results of all the possible combinations of the 3 assays performed with 4 different reagents were analyzed. Thirteen combinations of reagents allowed ≥ 80% of plasmas of each category (LA, factor deficiency or OAC) to be correctly classified (3/3 positive test results in LA-containing plasmas and 0/3 positive results in LA-negative samples).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b5ff361e5e2f9f462704511286080d3 https://doi.org/10.1055/s-0038-1655965 Zobrazit plný text záznamu