Analysis of lasalocid residues in grease and fat using liquid chromatography-mass spectrometry
| Autor: | Joseph M Storey, Susan B. Clark, Justin R. Carr, Mark R. Madson |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Lasalocid
Health Toxicology and Mutagenesis Syringe filter Ethyl acetate Industrial Waste Food Contamination Toxicology Mass Spectrometry Fats chemistry.chemical_compound Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Grease Solid phase extraction Chromatography Elution Solid Phase Extraction Public Health Environmental and Occupational Health General Chemistry General Medicine Animal Feed Drug Residues Streptomyces Anti-Bacterial Agents Triple quadrupole mass spectrometer chemistry Coccidiostats Oils Chromatography Liquid Food Science |
| Zdroj: | Food Additives & Contaminants: Part A. 32:1243-1248 |
| ISSN: | 1944-0057 1944-0049 |
| DOI: | 10.1080/19440049.2015.1052572 |
| Popis: | A method for the determination of lasalocid, an antibiotic and coccidiostat, in grease and fat is described. The manufacture of lasalocid produces a grease-like residue as a waste byproduct. Recently this byproduct has been shown to have been illegally introduced into the animal feed chain. Therefore, a quantitative and confirmatory procedure to analyse for lasalocid in this matrix is needed. A portion of grease/oil sample was extracted into hexane-washed acetonitrile, and a portion of the extract was then applied to a carboxylic acid solid-phase extraction (SPE) column for concentration and clean-up. The SPE column was washed with additional hexane-washed acetonitrile and ethyl acetate/methanol, after which lasalocid was eluted with 10% ammoniated methanol. The eluate was evaporated to dryness, redissolved in (1:1) acetonitrile-water and filtered through a PTFE syringe filter. Confirmation and quantitation of lasalocid in the final extract employed a triple quadrupole LC-MS/MS. The method was applied to grease and oil samples containing from 0.02 to 34,000 mg kg(-1) of lasalocid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |