Effect of simvastatin on the synthesis and secretion of lipoproteins in relation to the metabolism of cholesterol in cultured hepatocytes
| Autor: | Marise Mangeney, Claude Loriette, Dominique Pepin, Gilbert Bereziat, Brigitte Janvier, Agnès Ribeiro, Jean Chambaz, Ginette Thomas |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Apolipoprotein E
Male medicine.medical_specialty Very low-density lipoprotein Simvastatin Apolipoprotein B Lipoproteins Biophysics Biochemistry chemistry.chemical_compound Endocrinology Methionine Internal medicine medicine Animals Secretion Lovastatin Cells Cultured Triglycerides biology Cholesterol Fatty Acids Rats Inbred Strains Rats medicine.anatomical_structure chemistry Animals Newborn Liver Hepatocyte HMG-CoA reductase biology.protein lipids (amino acids peptides and proteins) Cholesterol Esters Hydroxymethylglutaryl-CoA Reductase Inhibitors medicine.drug |
| Zdroj: | Biochimica et biophysica acta. 1086(3) |
| ISSN: | 0006-3002 |
| Popis: | In primary culture of rat hepatocytes, simvastatin, a powerful HMGCoA reductase inhibitor, inhibited acetate incorporation into cellular and secreted cholesterol and cholesteryl-esters, without any significant effect on triacylglycerol synthesis and secretion. When applied to the culture for 24 h at 10 −7 M, a concentration shown to inhibit cholesterol synthesis by 61%, simvastatin increased apolipoprotein BH and BL synthesis and secretion and strongly decreased apolipoprotein AI synthesis and secretion whereas apolipoprotein AIV remained unaffected. The synthesis and secretion of apolipoprotein E was only slightly affected in contrast with other situations where cholesterol synthesis decreased. All of these modifications occurred at a post-transcriptional level, as the corresponding messenger RNAs of the apolipoproteins did not vary. These results suggest that either the drug itself or variations in cholesterol synthesis might be involved in apo B and apo AI synthesis and secretion. |
| Databáze: | OpenAIRE |
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