A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast
| Autor: | Daniele Novarina, Andriana Koutsoumpa, Andreas Milias-Argeitis |
|---|---|
| Přispěvatelé: | Molecular Systems Biology |
| Rok vydání: | 2022 |
| Předmět: |
Gene Editing/methods
Gene Editing Plasmids/genetics General Immunology and Microbiology General Neuroscience Saccharomycetales/genetics CRISPR-Cas Systems/genetics Saccharomyces cerevisiae General Biochemistry Genetics and Molecular Biology Saccharomyces cerevisiae/genetics ComputingMethodologies_PATTERNRECOGNITION Saccharomycetales CRISPR-Cas Systems Plasmids |
| Zdroj: | STAR protocols, 3(2):101358 |
| ISSN: | 2666-1667 |
| Popis: | CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.org/kits/moclo-ytk/). This step-by-step protocol guides the reader from sgRNA design to verification of the desired genome editing event and provides preassembled plasmids for cloning the sgRNA(s), making this technology easily accessible to any yeast research group. For complete details on the use and execution of this protocol, please refer to Novarina et al. (2021). |
| Databáze: | OpenAIRE |
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