Determination of di-and polyamines by high-performance liquid chromatographic separation of their 5-dimethyl aminonaphthalene-1-sulfonyl derivatives
| Autor: | Nikolaus Seiler, Bernd Knödgen, Friedhelm Eisenbeiss |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Dansyl Compounds
Sulfonyl chemistry.chemical_classification Chromatography Spermine Chick Embryo General Chemistry Diamines Spermidine Mice chemistry.chemical_compound Chromatographic separation chemistry Fully automated Methods Polyamines Animals Gradient elution Polyamine Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 145:29-39 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(00)81665-5 |
| Popis: | Using a Lichrosorb RP-8 reversed-phase column and a methanol—water gradient elution program, it is possible to separate within 40 min and to determine routinely in picomole quantities the natural di- and polyamines. The precision of the method is comparable to the thin-layer chromatographic procedures, the separations are most efficient, and the method can be fully automated. A modified gradient enables the repeated assay of spermidine and spermine within 20 min. The method is suited for polyamine analyses in tissues and body fluids. |
| Databáze: | OpenAIRE |
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