Malawian children with uncomplicated and cerebral malaria have decreased activated Vγ9Vδ2 γδ T cells which increase in convalescence

Autor: Karl B. Seydel, Kami Kim, Visopo Harawa, Madi Njie, Anthony Jaworowski, Stephen J. Rogerson, Thomas Keller, Wilson L. Mandala
Rok vydání: 2019
Předmět:
0301 basic medicine
Malawi
Cell
Lymphocyte Activation
Machine Learning
White Blood Cells
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
030212 general & internal medicine
Lymphocytes
Child
Immune Response
media_common
Protozoans
Multidisciplinary
medicine.diagnostic_test
T Cells
Convalescence
CD69
Malarial Parasites
Eukaryota
Receptors
Antigen
T-Cell
gamma-delta
3. Good health
medicine.anatomical_structure
Treatment Outcome
Cerebral Malaria
Child
Preschool
Medicine
Cellular Types
Research Article
Science
media_common.quotation_subject
T cell
Immune Cells
Plasmodium falciparum
Immunology
Cell Enumeration Techniques
Malaria
Cerebral
Research and Analysis Methods
Flow cytometry
03 medical and health sciences
medicine
Parasitic Diseases
Humans
Secondary lymphoid tissue
Lymphocyte Count
Blood Cells
business.industry
Organisms
Infant
Biology and Life Sciences
Cell Biology
medicine.disease
Tropical Diseases
Parasitic Protozoans
Malaria
Health Care
030104 developmental biology
Case-Control Studies
business
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 10, p e0223410 (2019)
ISSN: 1932-6203
Popis: Malaria is responsible for almost half a million deaths annually. The role of Vγ9Vδ2 γδ T cells in malaria is still unclear. Studies have reported an association between this cell subset and malaria symptoms and severity. Profiles of Vγ9Vδ2 γδ T cells in bigger cohorts with different levels of clinical severity have not been described. Proportion, numbers, and activation status of Vγ9Vδ2 γδ T cells were measured by flow cytometry in 59 healthy controls (HCs), 58 children with uncomplicated malaria (UM) and 67 with cerebral malaria (CM,) during acute malaria and in convalescence 28 days later. Vγ9Vδ2 γδ T cell were lower in children presenting with UM and CM than in HCs. Cell counts did not vary with malaria severity (CM median counts 40 x 103 cells/μL, IQR [23-103]; UM median counts 30 x 103 cells/μL [10-90], P = 0.224). Vγ9Vδ2 γδ T cell counts increased during convalescence for UM (70 [40-60] x 103 cells/μL and CM (90 [60-140] x 103 cells/μL), to levels similar to those in HCs (70 [50-140] x 103 cells/μL), p = 0.70 and p = 0.40 respectively. Expression of the activation markers CD69 and HLA-DR on Vγ9Vδ2 γδ T cells was higher in malaria cases than in controls (HCs vs UM or CM, p < 0.0001) but was similar between UM and CM. HLA-DR expression remained elevated at 28 days, suggesting sustained activation of Vγ9Vδ2 γδ T cells during recovery. Vγ9Vδ2 γδ T cell proportions and cells counts were suppressed in acute disease and normalized in convalescence, a phenomenon previously hypothesized to be due to transient migration of the cells to secondary lymphoid tissue. The presence of highly activated Vγ9Vδ2 γδ T cells suggests that this T cell subset plays a specific role in response to malaria infection.
Databáze: OpenAIRE
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