Lineage II (Serovar 1/2a and 1/2c) HumanListeria monocytogenesPulsed-Field Gel Electrophoresis Types Divided into PFGE Groups Using the Band Patterns Below 145.5 kb
| Autor: | Richard V. Goering, Gloria Lopez-Valladares, Wilhelm Tham, Marie-Louise Danielsson-Tham |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Serotype Lineage (genetic) 030106 microbiology Food Contamination Biology Serogroup medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Disease Outbreaks Foodborne Diseases 03 medical and health sciences Listeria monocytogenes medicine Pulsed-field gel electrophoresis Humans Listeriosis Serotyping Sweden Gel electrophoresis fungi Molecular biology Electrophoresis Gel Pulsed-Field 030104 developmental biology Food Microbiology Animal Science and Zoology Multilocus Sequence Typing Food Science |
| Zdroj: | Foodborne Pathogens and Disease. 14:8-16 |
| ISSN: | 1556-7125 1535-3141 |
| Popis: | Among 504 clinical lineage II isolates of Listeria monocytogenes isolated during 1958-2010 in Sweden, 119 pulsed-field gel electrophoresis (PFGE) types (AscI) have been identified based on the number and distribution of all banding patterns in each DNA profile. In this study, these types were further divided into PFGE groups based on the configuration of small bands with sizes145.5 kb. The 504 isolates included 483 serovar 1/2a isolates distributed into 114 PFGE types and 21 serovar 1/2c isolates distributed into 9 PFGE types; these were further divided into 21 PFGE groups. PFGE group, that is, configuration of small bands below 145.5 kb, and serovars were correlated. L. monocytogenes isolates belonging to PFGE groups A, B, C, E, F, H, K, L, M, S, V, W, Y, and Ö-6 to Ö-12 shared serovar 1/2a, with one exception. PFGE group E also included two PFGE types sharing serovar 1/2c and four PFGE types belonging to either serovar 1/2a or 1/2c. Isolates belonging to PFGE group N shared serovar 1/2c. In contrast to lineage I isolates, small fragments33.3 kb were visible in all L. monocytogenes isolates belonging to lineage II. In the results from both the present and previous studies, the genomic region of small bands was genetically more conservative than in large bands. The distribution of these small bands established the relatedness of strains and defined a genetic marker for both lineages I and II, while also establishing their serogroup. The division of L. monocytogenes PFGE types into PFGE groups is advantageous as the profile of every new isolate can be identified easily and quickly through first studying the PFGE group affiliation of the isolate based on the smaller band patterns145.5 kb, and then identifying the PFGE type based on the band patterns145.5 kb. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|